- Clone
- S15049F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- M130, MM130, SCARI1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S15049F_PE-Dazzle-594_CD163_Antibody_090622 S15049F_PE-Dazzle-594_CD163_Antibody_090622](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S15049F_PE-Dazzle-594_CD163_Antibody_090622-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were treated with True-Stain Monocyte Blocker™. Cells were then stained with anti-mouse CD11b APC and anti-mouse CD163 (clone S15049F) PE/Dazzle™ 594 (left), or rat IgG2a, κ PE/Dazzle™ 594 isotype control (right). Data shown was gated on total bone marrow cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
156709 | 25 µg | £93 | ||||
156710 | 100 µg | £243 |
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD163 extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2927871 (BioLegend Cat. No. 156709)
AB_2927871 (BioLegend Cat. No. 156710)
Antigen Details
- Structure
- Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
- Distribution
-
Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells
- Function
- Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages.
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- 93671 View all products for this Gene ID
- UniProt
- View information about CD163 on UniProt.org
Other Formats
View All CD163 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD163 | S15049F | FC |
PE anti-mouse CD163 | S15049F | FC |
APC anti-mouse CD163 | S15049F | FC |
PE/Cyanine7 anti-mouse CD163 | S15049F | FC |
PE/Dazzle™ 594 anti-mouse CD163 | S15049F | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD163
C57BL/6 bone marrow cells were stained with CD11b APC and pu... -
PE anti-mouse CD163
C57BL/6 bone marrow cells were stained with CD11b APC and CD... -
APC anti-mouse CD163
C57BL/6 bone marrow cells were stained with anti-mouse/human... -
PE/Cyanine7 anti-mouse CD163
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Dazzle™ 594 anti-mouse CD163
C57BL/6 mouse bone marrow cells were treated with True-Stain...
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