- Clone
- MIH43 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- VTCN1, B7h.5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MIH43_PerCPCy5.5_B7H4_Antibody_020314 MIH43_PerCPCy5.5_B7H4_Antibody_020314](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MIH43_PerCPCy5.5_B7H4_Antibody_020314.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human B7-H4 transfected P815 cells were stained with B7-H4 (clone MIH43) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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358110 | 100 tests | £262 |
B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Application Notes
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Additional reported applications (for the relevant formats) includes immunofluorescence2.
- Additional Product Notes
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BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Quandt D, et al. 2011. Clin. Cancer Res. 17:3100.
- Smith JB, et al. 2014. Gynecol. Oncol. 134:181. (FC, IF)
- RRID
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AB_2563122 (BioLegend Cat. No. 358110)
Antigen Details
- Structure
- Type I transmembrane protein, member of the B7 family, one IgV-like, one IgC-like domain
- Distribution
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Activated T cells, B cells, monocytes, and dendritic cells; different cancer types including melanoma, renal, gastric, breast and ovarian
- Function
- Inhibits T cell proliferation, cytokine secretion, and development of cytotoxicity
- Cell Type
- B cells, Dendritic cells, Monocytes, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Antigen References
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1. Fauci JM, et al. 2012. Gynecol. Oncol. 127:420.
2. Chen C, et al. 2012. J. Immunother. 35:354.
3. Guo G, et al. 2012. Clin. Rheumatol. 31:271.
4. Arigami T, et al. 2010. J. Surg. Oncol. 102:748. - Gene ID
- 79679 View all products for this Gene ID
- UniProt
- View information about B7-H4 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All B7-H4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human B7-H4 | MIH43 | FC,IHC-F,ICC |
PE anti-human B7-H4 | MIH43 | FC |
PE/Cyanine7 anti-human B7-H4 | MIH43 | FC |
APC anti-human B7-H4 | MIH43 | FC |
PerCP/Cyanine5.5 anti-human B7-H4 | MIH43 | FC |
PE/Dazzle™ 594 anti-human B7-H4 | MIH43 | FC |
TotalSeq™-A0395 anti-human B7-H4 | MIH43 | PG |
TotalSeq™-C0395 anti-human B7-H4 | MIH43 | PG |
APC/Fire™ 750 anti-human B7-H4 | MIH43 | FC |
TotalSeq™-B0395 anti-human B7-H4 | MIH43 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with purifie... -
PE anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with B7-H4 (... -
PE/Cyanine7 anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with B7-H4 (... -
APC anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with B7-H4 (... -
PerCP/Cyanine5.5 anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with B7-H4 (... -
PE/Dazzle™ 594 anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with B7-H4 (... -
TotalSeq™-A0395 anti-human B7-H4
-
TotalSeq™-C0395 anti-human B7-H4
-
APC/Fire™ 750 anti-human B7-H4
Human B7-H4 transfected P815 cells were stained with human B... -
TotalSeq™-B0395 anti-human B7-H4
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