- Clone
- S18015E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B1, Bp35
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S18015E_PerCPCyanine5_CD20_Antibody_020821 S18015E_PerCPCyanine5_CD20_Antibody_020821](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S18015E_PerCPCyanine5_CD20_Antibody_020821.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human CD20 (clone S18015E) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
375509 | 25 tests | £73 | ||||
375510 | 100 tests | £201 |
CD20 (also known as B1 and Bp35) is a four transmembrane spanning protein measuring 33-37 kD. Expressed on pre-B cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. Involved in B-cell activation and is associated with a variety of Src family kinases.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD20 transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
- RRID
-
AB_2890823 (BioLegend Cat. No. 375509)
AB_2890823 (BioLegend Cat. No. 375510)
Antigen Details
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Interaction
- The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn).
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Hultin LE, et al. 1993. Cytometry 14:196.
- Tedder T and Engel P. 1994. Immunol. Today 15:450.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD20 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD20 | S18015E | FC |
PE anti-human CD20 Antibody | S18015E | FC |
APC anti-human CD20 Antibody | S18015E | FC |
PerCP/Cyanine5.5 anti-human CD20 Antibody | S18015E | FC |
FITC anti-human CD20 Antibody | S18015E | FC |
PE/Dazzle™ 594 anti-human CD20 | S18015E | FC |
Brilliant Violet 421™ anti-human CD20 | S18015E | FC |
Pacific Blue™ anti-human CD20 | S18015E | FC |
PE/Cyanine7 anti-human CD20 | S18015E | FC |
APC/Fire™ 750 anti-human CD20 | S18015E | FC |
Spark UV™ 387 anti-human CD20 | S18015E | FC |
APC/Fire™ 810 anti-human CD20 | S18015E | FC |
Alexa Fluor® 700 anti-human CD20 | S18015E | FC |
PerCP anti-human CD20 | S18015E | FC |
Alexa Fluor® 647 anti-human CD20 | S18015E | FC |
Spark Violet™ 500 anti-human CD20 | S18015E | FC |
PE/Fire™ 810 anti-human CD20 | S18015E | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD20
Human peripheral blood lymphocytes were surface stained with... -
PE anti-human CD20 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD20 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD20 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD20 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Dazzle™ 594 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
Pacific Blue™ anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 750 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark UV™ 387 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 700 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 647 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Violet™ 500 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu...
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