- Clone
- 1H9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Paired box 5, B-cell lineage specific activator, BSAP, paired box homeotic gene 5
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1H9_PerCPCyanine55_PAX-5_Antibody_1_010219 1H9_PerCPCyanine55_PAX-5_Antibody_1_010219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1H9_PerCPCyanine55_PAX-5_Antibody_1_010219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with mouse CD3 APC then fixed, permeabilized and intracellularly stained with Pax-5 (clone 1H9) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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649709 | 25 µg | £121 | ||||
649710 | 100 µg | £282 |
Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (Pax) family of transcription factors. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. Pax5 is the only member of the Pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis.
This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse Pax-5 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Application Notes
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The monoclonal 1H9 antibody recognizes both mouse and human Pax5.
Additional reported applications (for the relevant formats) include: Western blotting1,2, immunohistochemical staining of formalin-fixed sections, and spatial biology (IBEX)4,5.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2562572 (BioLegend Cat. No. 649709)
AB_2562573 (BioLegend Cat. No. 649710)
Antigen Details
- Structure
- 391 amino acids with predicted molecular weight of 42 kD
- Distribution
-
Nucleus
- Function
- Plays an important role in B-cell differentiation as well as neural development and spermatogenesis; involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene
- Interaction
- Interacts with DAXX; binds DNA as a monomer, binds TLE4
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Liao F, et al. 1992. J. Immunol. 148:2909.
2. Nutt SL, et al. 1997. Genes Dev. 11:476.
3. Nera KP, et al. 2006. Immunity 24:283.
4. Fuxa M, et al. 2007. Immunity 178:3031. - Gene ID
- 5079 View all products for this Gene ID
- UniProt
- View information about Pax-5 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All Pax-5 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Pax-5 | 1H9 | WB,ICC,IHC-P |
Alexa Fluor® 647 anti-Pax-5 | 1H9 | ICFC,SB |
Alexa Fluor® 488 anti-Pax-5 | 1H9 | ICFC |
PE anti-Pax-5 | 1H9 | ICFC |
PerCP/Cyanine5.5 anti-Pax-5 | 1H9 | ICFC |
Alexa Fluor® 594 anti-Pax-5 | 1H9 | ICC |
TotalSeq™-Bn1223 anti-Pax-5 | 1H9 | SB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Pax-5
Western blot analysis of Daudi (lane 1) and A20 (lane 2) cel... Ramos cells were fixed with 4% paraformaldehyde (PFA) for te... Tissue from human tonsil sections was fixed in 10% buffered ... -
Alexa Fluor® 647 anti-Pax-5
C57BL/6 mouse splenocytes were surface stained with mouse CD... Mice were injected subcutaneously with sheep red blood cells... -
Alexa Fluor® 488 anti-Pax-5
C57BL/6 mouse splenocytes were surface stained with mouse CD... -
PE anti-Pax-5
C57BL/6 mouse splenocytes were surface stained with mouse CD... -
PerCP/Cyanine5.5 anti-Pax-5
C57BL/6 mouse splenocytes were stained with mouse CD3 APC th... -
Alexa Fluor® 594 anti-Pax-5
Daudi cells were fixed with 4% paraformaldehyde (PFA) for 10... -
TotalSeq™-Bn1223 anti-Pax-5
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