- Clone
- BL28596 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- a1AGP, ORM1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119 BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 5 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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859601 | 25 µg | £73 |
α-1-Acid Glycoprotein (α1AGP) is a key acute phase plasma protein synthesized by hepatocytes and functions as a transport protein in the blood stream. The levels of α1AGP become elevated in acute inflammation and therefore it is classified as an acute-phase reactant.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant α-1-Acid Glycoprotein (a1AGP) expressed in E.coli.
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810733 (BioLegend Cat. No. 859601)
Antigen Details
- Structure
- α-1-Acid Glycoprotein is a 201 amino acid protein with a predicted molecular mass of 24 kD and observed molecular mass of 40 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus - Function
- α-1-Acid Glycoprotein is an acute phase plasma protein which functions as transport protein in the blood stream.
- Interaction
- Synthetic drugs, cytosolic Ca2+, immune response
- Cell Type
- Mesenchymal cells, Neurons
- Biology Area
- Immuno-Oncology, Immunology
- Molecular Family
- Adaptor Proteins, Innate Immune Signaling
- Antigen References
-
- Luo Z, et al. 2015. J Physiol Biochem. 71(2):329.
- Xu YF, et al. 2018. Comb Chem High Throughput Screen. 21(10):755.
- Gene ID
- 5004 View all products for this Gene ID 18405 View all products for this Gene ID 24614 View all products for this Gene ID
- UniProt
- View information about alpha-1-Acid Glycoprotein on UniProt.org
Other Formats
View All α-1-Acid Glycoprotein Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-α-1-Acid Glycoprotein | BL28596 | WB,IHC-P |
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