- Clone
- N388A/10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ANK1, Ankyrin-R
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N388A-10_Purified_Ankyrin-R_Antibody_1_112018 N388A-10_Purified_Ankyrin-R_Antibody_1_112018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N388A-10_Purified_Ankyrin-R_Antibody_1_112018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Ankyrin-R antibody (clone N388A/10). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
Western blot of purified anti-Ankyrin-R antibody (clone N388A/10). Lane 1: Molecular weight marker; Lane 2: 40 µg of human cerebellum lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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857701 | 25 µg | £57 | ||||
857702 | 100 µg | £113 |
Ankyrin-R is an anchor protein whose main role in vivo is to anchor integral membrane protein/complexes to the cytoskeleton. It was originally discovered in erythrocytes, but later to be found expressed in the brain. The gene encoding Ankyrin-R, ANK1, has a highly complex regulatory mechanism, which gives rise to a variety of different isoforms of Ankyrin-R. Recent studies in Alzheimer’s Disease have shown that epigenetic dysregulation of ANK1, in combination of several other proteins including Trem-2, could be a contributing factor of increased TNFA expression and neuroinflammation1.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Taylor A, et al. 2017. Front Cell Neurosci. 11:11 (IHC-F)
- Saifetiarova J, et al. 2017. J Neurosci. 37(10):2524 (IHC-F)
- Taylor A, et al. 2018. eNeuro. 5(3) (IHC-F, WB)
- RRID
-
AB_2783477 (BioLegend Cat. No. 857701)
AB_2783478 (BioLegend Cat. No. 857702)
Antigen Details
- Structure
- ANK1 is a 1881 amino acid protein with an apparent molecular mass of ~ 206 kD.
- Distribution
-
Tissue Distribution: Brain and bone marrow.
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, nucleus, and endoplasmic reticulum. - Function
- ANK1 functions as an adaptor protein to anchor integral membrane proteins/complexes to the cytoskeleton.
- Interaction
- SPTB, SLC4A1, EPB41, TTN, OBSCN, HIF1AN
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adaptor Proteins
- Antigen References
-
- Morris G, et al. 2018. Mol Neurobiol. doi: 10.1007/s12035-018-1092-y.
- Nicolas V, et al. 2006. Transfuse Clin Biol. 13(1-2):23
- Kline CF, et al. 2006. Expert Rev Cardiovasc Ther. 4(4):477
- Gene ID
- 286 View all products for this Gene ID 11733 View all products for this Gene ID 306570 View all products for this Gene ID
- UniProt
- View information about Ankyrin-R on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Ankyrin-R Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Ankyrin-R | N388A/10 | WB |
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