- Clone
- Poly28616 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GOT1, Aspartate Aminotransferase
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly28616_Purified_Aspartate-Aminotransferase_Antibody_1_050119 Poly28616_Purified_Aspartate-Aminotransferase_Antibody_1_050119](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly28616_Purified_Aspartate-Aminotransferase_Antibody_1_050119.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Aspartate Aminotransferase antibody (Poly28616). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Aspartate Aminotransferase antibody (Poly28616) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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861601 | 25 µg | £86 |
GOT1 is the cytoplasmic form of aspartate aminotransferase and plays a role in amino acid metabolism, and the tricarboxylic acid cycles. GOT1 is one of the 6 genes that is commonly shared and regulated in the aging population and AD patients, suggesting that it may contribute as an aging-dependent AD risk factor1.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant Aspartate Aminotransferase (AST)
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2810746 (BioLegend Cat. No. 861601)
Antigen Details
- Structure
- GOT1 is a 413 amino acid protein with an apparent molecular mass of ~ 46 kD.
- Distribution
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Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus - Function
- GOT1 is a cytoplasmic aspartate aminotransferase that plays a role in amino acid metabolism and the tricarboxylic acid cycles.
- Interaction
- Forms homodimer.
- Cell Type
- Astrocytes, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
- Biology Area
- Neurodegeneration, Neuroinflammation, Neuroscience
- Antigen References
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- Zheng JJ, et al. 2018. Medicine (Baltimore). 97(28):e11343
- Zhang K, et al. 2018. Mol Carcinog. 57(11):1566
- Gene ID
- 2805 View all products for this Gene ID
- UniProt
- View information about Aspartate Aminotransferase on UniProt.org
Other Formats
View All Aspartate Aminotransferase Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Aspartate Aminotransferase | Poly28616 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Aspartate Aminotransferase
Western blot of purified anti-Aspartate Aminotransferase ant... IHC staining of purified anti-Aspartate Aminotransferase ant...
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