- Clone
- A20145B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, oncogene NGL, neuroblastoma- or glioblastoma-derived, HER-2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a. A20145B_PURE_CD340_ICC_070723 a. A20145B_PURE_CD340_ICC_070723](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A20145B_PURE_CD340_ICC_070723.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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SK-BR-3 cells were fixed with 4% Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5.0 µg/mL of purified anti-CD340 (ErbB2/HER-2) (clone A20145B), followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B). Images were captured with a 60X objective. Scale bar: 50 µm -
Human breast cancer cell line (SKBR3) stained with purified anti-CD340 (ErbB2/HER-2) (clone A20145B) (filled histogram) or purified mouse IgG2b, κ (open histogram), followed by PE anti-mouse IgG1 (Cat. No. 406607).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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616151 | 25 µg | £126 | ||||
616152 | 100 µg | £315 |
CD340 is the protein HER2, a member of the epidermal growth factor receptor (EGFR) family of cell membrane tyrosine kinases, and is encoded by the gene ErbB2. Unlike other EGFR family members, CD340 contains no ligand binding domain and has no known ligands. Instead, this protein interacts with other EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of ErbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of human ErbB2, N-terminus
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
FC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product is not recommended for use in IHC-P.
- RRID
-
AB_3068205 (BioLegend Cat. No. 616151)
AB_3068205 (BioLegend Cat. No. 616152)
Antigen Details
- Structure
- CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kD.
- Distribution
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Expressed on the cell surface of many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, glioblastoma, some c-ALL blasts and bone marrow MSCs
- Function
- Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
- Interaction
- CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin-1, JAK2, PAK1, FAK, and β-catenin, among others.
- Ligand/Receptor
- Despite being a member of the epidermal growth factor receptor (EGFR) family, no ligand of CD340 has been identified.
- Cell Type
- Leukemia
- Biology Area
- Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Akiyama T, et al. 1986. Science. 232:1644-6.
- Bargmann CI, et al. 1986. Nature. 319:226-30.
- Pegram MD, et al. 1997. Oncogene. 15:537-47.
- Slamon DJ, et al. 1989. Science. 244:707-12.
- Leone F, et al. 2003. J Leukocyte Biol. 74:593-601.
- Gene ID
- 2064 View all products for this Gene ID
- UniProt
- View information about CD340 on UniProt.org
Other Formats
View All CD340 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CD340 (ErbB2/HER-2) | A20145B | ICC,FC |
Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2) | A20145B | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-CD340 (ErbB2/HER-2)
SK-BR-3 cells were fixed with 4% Fixation Buffer (Cat. No. 4... Human breast cancer cell line (SKBR3) stained with purified ... -
Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2)
Human breast cancer cell line (SK-BR-3) was stained with ant...
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