- Clone
- W20081E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Dynamin-1-like protein (DNM1L), Dnm1p/Vps1p-like protein (DVLP), Dynamin family member proline-rich carboxyl-terminal domain less (DYMPLE), Dynamin-like protein 1 (DLP1), Dynamin-related protein 1 (DRP1)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W20081E_PURE_DNM1L_Antibody_032822 W20081E_PURE_DNM1L_Antibody_032822](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W20081E_PURE_DNM1L_Antibody_032822.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-DNM1L (clone W20081E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
ICC staining of purified anti-DNM1L (clone W20081E) on HeLa cells. The cells were fixed and permeabilized with ice cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 60X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
603851 | 25 µg | £81 | ||||
603852 | 100 µg | £201 |
Dynamin-1-like protein (DNM1L) is a member of the dynamin superfamily of GTPases. Its best studied role is in the control of mitochondrial fission. DNM1L oligomerizes into filaments that form rings around the scission site to “pinch off” dividing mitochondria using GTP hydrolysis. Accordingly, DNM1L also has important functions in the mitochondrial dynamics of cell proliferation and apoptosis. DNM1L is also required for proper formation of the nervous system, and deficiencies in its functions have been linked to Alzheimer’s Disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human DNM1L protein fragments
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For Western Blot, higher molecular weight band (≈100 kDa) likely corresponds to SUMOylated DNM1L (e.g. Figueroa-Romer, et al. 2009).
For ICC, we recommend fixation/permeabilization with 100% ice cold methanol alone. Alternatively, 4% paraformaldehyde fixation followed by permeabilization with ice cold methanol is possible. We do not recommend 4% paraformaldehyde fixation followed by permeabilization with 0.5% Triton-X, due to incorrect signal localization.
Not recommended for use in IHC-P (human). - RRID
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AB_2922636 (BioLegend Cat. No. 603851)
AB_2922636 (BioLegend Cat. No. 603852)
Antigen Details
- Structure
- DNM1L is a 736 amino acid protein with a predicted molecular weight of 81.8 kD.
- Distribution
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DNM1L is widely expressed in the cytoplasm, mitochondria, and peroxisomes of most tissues.
- Function
- DNM1L is a GTPase
- Interaction
- CDK1
- Biology Area
- Cell Motility/Cytoskeleton/Structure, Mitochondrial Function
- Molecular Family
- Cytoskeletal Proteins, GTPases
- Antigen References
-
- Oliver D and Reddy PH. 2019. Cells. 8:961.
- Reddy PH, et al. 2011. Brain Res Rev. 67:103-18.
- Gene ID
- 10059 View all products for this Gene ID
- UniProt
- View information about DNM1L on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All DNM1L Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-DNM1L | W20081E | WB,ICC |
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