- Clone
- 6RN3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- rpoN, glnF, ntrA, RNA Polymerase
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6RN3_PURE_E-coli_RNA_Sigma_54_Antibody_101019.png 6RN3_PURE_E-coli_RNA_Sigma_54_Antibody_101019.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6RN3_PURE_E-coli_RNA_Sigma_54_Antibody_101019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Whole cell extracts (15 µg protein) from DH5α E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-E. coli RNA Sigma 54 Antibody, clone 6RN3, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
663305 | 25 µg | £70 | ||||
663306 | 100 µg | £174 |
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and then are released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (Sigma 54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein glnG (ntrC).
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Previously item# W0005 from Neoclone.
Clone 6RN3 recognizes epitope 88-137.
Additional reported applications (for the relevant formats) include: functional assay.1 -
Application References
(PubMed link indicates BioLegend citation) -
- Zhao K, et al. 2010. Nucleic Acids Res. 38:1273. (FA)
- RRID
-
AB_2820108 (BioLegend Cat. No. 663305)
AB_2820109 (BioLegend Cat. No. 663306)
Antigen Details
- Structure
- Sigma 54 (σ54) is a 54 kD transcription factor of E. coli RNA polymerase. Observed molecular weight is around 70 kD.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 947714 View all products for this Gene ID
- UniProt
- View information about Sigma 54 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Sigma 54 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-E. coli RNA Sigma 54 | 6RN3 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-E. coli RNA Sigma 54
Whole cell extracts (15 µg protein) from DH5α E. coli...
Follow Us