- Clone
- N87/25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Gamma-aminobutyric acid receptor subunit beta-3, cleft palate 1, GABA(A) receptor subunit beta-3, gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3
- Previously
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Covance Catalog# MMS-5134
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N87slash25_PUREAF_GABRB3_Antibody_1_WB_020518 N87slash25_PUREAF_GABRB3_Antibody_1_WB_020518](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N87slash25_PUREAF_GABRB3_Antibody_1_WB_020518.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-GABRB3 antibody (clone N87/25). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate; Lane 5: 20 µg Jurkat cell lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-GABRB3 antibody (clone N87/25) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-GABRB3 antibody (clone N87/25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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818501 | 100 µL | £113 |
Gamma-aminobutyric acid receptor subunit beta-3 (GABRB3) is a component of the hetero-pentameric receptor for gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate brain. The receptor is a ligand-gated multi-subunit chloride channel. The GABRB3 protein is encoded by the GABRB3 gene in humans. Mutations in this gene have been associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, epilepsy and autism.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 370-433 of mouse GABA(A) Receptor, Beta-3 protein.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2564821 (BioLegend Cat. No. 818501)
Antigen Details
- Structure
- Expected Molecular Weight: 55 kD.
- Cell Type
- GABAergic Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 14402 View all products for this Gene ID
- UniProt
- View information about GABRB3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GABA(A)R, β3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GABRB3 | N87/25 | WB,IHC-P |
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