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Purified anti-GD3 (MEL-1) Recombinant Antibody

Pricing & Availability
Clone
R24.Rec (See other available formats)
Regulatory Status
RUO
Other Names
GD3S, SIAT8, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, SIAT8-A, ganglioside GD3 synthase, sialytransferase St8Sia I, alpha-2, 8-sialyltransferase 8A, disialoganglioside (GD3) synthase, ganglioside-specific alpha-2, 8-polysialyltransferase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. R24rec_PURE_GD3_-IHC-F_061124
IHC staining of Purified anti-GD3 (MEL-1) (clone R24.Rec) on frozen melanoma (panels A and B) and adjacent normal skin (panels C and D) tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated without (panels A and C) and with (panels B and D) primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining. Images were captured with a 10X objective and merged. Scale bar: 50 µm
  • A. R24rec_PURE_GD3_-IHC-F_061124
    IHC staining of Purified anti-GD3 (MEL-1) (clone R24.Rec) on frozen melanoma (panels A and B) and adjacent normal skin (panels C and D) tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated without (panels A and C) and with (panels B and D) primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining. Images were captured with a 10X objective and merged. Scale bar: 50 µm
  • B. R24rec_PURE_GD3_-ICC_061124
    SK-MEL-28 cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were stained with Purified mouse IgG1, κ isotype control (Cat. No. 400101) (panel A) or stained with Purified anti-GD3 (MEL-1) (clone R24.Rec) (panel B), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) (red) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Flash Phalloidin™ Green 488 was used to visualize cytoskeleton (Cat. No. 424201) (green). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. R24rec_PURE_GD3_-ICFC_061124
    SK-Mel-28 cells (positive control, filled histogram) and HeLa cells (low expression control, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat No. 426803) and intracellularly stained with Purified anti-GD3 (MEL-1) (clone R24.Rec), or purified mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat No. 400102).
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638851 25 µg £117
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638852 100 µg £305
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Description

GD3, also known as MEL-1, is a ganglioside found in almost all melanomas and is an important biomarker for malignant melanoma. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. Anti-GD3 antibodies have been shown to induce partial remission of tumor growth due to the proliferation of cytotoxic lymphocytes. GD3 is synthesized by the enzyme ST8SIA1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
This antibody was raised against SK-MEL-28 melanoma cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone R24.Rec) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801), followed by permeabilization with 0.5% Triton-X. We do not recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with ice-cold methanol, nor fixation/permeabilization with ice-cold methanol alone. 

For use of this antibody (clone R24.Rec) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). 

This antibody (clone R24.Rec) is not suitable for use in immunohistochemistry with formalin-fixed, paraffin-embedded tissues (IHC-P).

Antigen Details

Structure
A ganglioside is comprised of a glycosphingolipid and one or more sialic acid groups.
Distribution

Cytoplasmic, ER/Golgi, secreted

Function
Cellular recognition, cell/cell signaling
Biology Area
Cancer Biomarkers
Antigen References
  1. Meidenbauer N, et al. 2004. Cancer Res. 64: 6319-26.
  2. Kasahara K, et al. 2000. J Biol Chem. 275: 34701-9.
  3. Watarai S, et al. 1991. J Biochem. 110:889-95.
  4. Yuyama K, et al. 2007. J. Biol. Chem. 282: 26392-400.
Gene ID
NA
UniProt
View information about GD3 on UniProt.org
Go To Top Version: 1    Revision Date: 06/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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