- Clone
- NGFR5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tumor necrosis factor receptor superfamily member 16, p75 ICD, NGF receptor, TNFR superfamily, member 16, low affinity nerve growth factor receptor, low affinity neurotrophin receptor p75NTR, CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR
- Previously
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Covance Catalog# MMS-5228
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![NGFR5_Purified_CD271_p75NTR_Antibody_IHC_030415 NGFR5_Purified_CD271_p75NTR_Antibody_IHC_030415](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/NGFR5_Purified_CD271_p75NTR_Antibody_IHC_030415.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-human CD271 (p75NTR) antibody (clone NGFR5) on formalin-fixed paraffin-embedded normal human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
IHC staining of purified anti-human CD271 (p75NTR) antibody (clone NGFR5) on formalin-fixed paraffin-embedded normal human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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818201 | 100 µL | £189 |
CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis and to regulate cell differentiation and neurogenesis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against A875 human myeloma cells and p75NTR purified from A875 human myeloma cells.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
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IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2564818 (BioLegend Cat. No. 818201)
Antigen Details
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 4804 View all products for this Gene ID
- UniProt
- View information about CD271 on UniProt.org
Related FAQs
Other Formats
View All CD271 (p75NTR) Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human CD271 (p75NTR) | NGFR5 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD271 (p75NTR)
IHC staining of purified anti-human CD271 (p75NTR) antibody ... IHC staining of purified anti-human CD271 (p75NTR) antibody ...
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