Purified anti-human HLA-DM Antibody

Pricing & Availability
Clone
MaP.DM1 (See other available formats)
Regulatory Status
RUO
Other Names
HLADM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Map.Dm1_Purified_HLA-DM_Antibody_FC_043013
Human peripheral blood lymphocytes were fixed, permeabilized, and then stained with purified HLA-DM (clone MaP.DM1, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • Map.Dm1_Purified_HLA-DM_Antibody_FC_043013
    Human peripheral blood lymphocytes were fixed, permeabilized, and then stained with purified HLA-DM (clone MaP.DM1, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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358002 100 µg £130
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Description

HLA-DM is a non-classical MHC molecule, comprised of one α chain and one β chain, which is expressed by antigen presenting cells. HLA-DM acts as a chaperon that stabilizes the MHC class II molecule in the lyzosome, while enhances the exchange of CLIP for the antigenic peptide.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MHC class II enriched organelles from the B cell line Pala.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hammond C, et al. 1998. J. Immunol. 161:3282.
  2. Faubert A, et al. 2002. J. Biol. Chem. 277:2750.
  3. Xiu F, et al. 2011. J. Immunol. 187:1547.
Product Citations
  1. Duarte JH, et al. 2013. PLoS One. e79819:8. PubMed
RRID
AB_2562026 (BioLegend Cat. No. 358002)

Antigen Details

Structure
Heterodimer comprising one α and one β chain.
Distribution
Antigen presenting cells.
Function
Facilitates the exchange of CLIP for the antigenic peptide in the grove of the MHC class II molecule, maintaining the tertiary structure of this molecule during the process.
Interaction
MHC class II
Cell Type
Antigen-presenting cells, B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3108 View all products for this Gene ID 3109 View all products for this Gene ID
UniProt
View information about HLA-DM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/01/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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