- Clone
- N201/35 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RING-type E3 Ubiquitin Transferase RNF146, Iduna. Ring Finger Protein 146
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N201slash35_PURE_Iduna_RNF146_Antibody_102021 N201slash35_PURE_Iduna_RNF146_Antibody_102021](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N201slash35_PURE_Iduna_RNF146_Antibody_102021.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole tissue membrane cell extracts (15 µg total protein) from human and rat brain were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-Iduna (RNF146) antibody (clone N201/35) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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602052 | 100 µg | £113 |
Ring finger protein 146 (RNF146), also known as Iduna, is a poly-ADP-ribose (PAR)-dependent E3 ubiquitin-protein ligase that promotes the degradation of PARsylated proteins. The ability of RNF146 to bind to PAR and thus inhibit PAR dependent cell death, or parthanatos, contributes to its role in DNA repair and cell survival and overall neuroprotective qualities. RNF146 has been identified as an NMDA receptor-induced survival gene that protects against NMDA receptor mediated excitotoxicity and ischemia-induced neuronal injury. RNF146 is a positive regulator of the Wnt/ β-catenin signaling pathway through the ubiquitylation of AXIN following its degradation by tankyrase and has been implicated in embryonic and postnatal osteoblast development, bone remodeling, and energy metabolism via coordination of β-catenin signaling and Hippo pathways within osteoblasts.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length mouse Iduna (RNF146) protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone has been tested for IHC and failed.
- RRID
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AB_2904447 (BioLegend Cat. No. 602052)
Antigen Details
- Structure
- RNF146 is a 359 amino acid protein with a predicted molecular weight of 39 kD.
- Distribution
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Ubiquitously expressed /Nucleus and Cytosol
- Function
- Wnt signaling pathway, Ubiquitin conjugation pathway
- Antigen References
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- Andrabi S, et al. 2011. Nat. Med. 17:692.
- Kang H, et al. 2011. PNAS. 108:14103.
- Matsumoto Y, et al. 2017. J. Clin. Invest. 127:2612.
- Zhang Y, et al. 2011. Nat. Cell Biol. 13:623.
- Gene ID
- 81847 View all products for this Gene ID
- UniProt
- View information about Iduna on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Iduna Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Iduna (RNF146) Antibody | N201/35 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Iduna (RNF146) Antibody
Whole tissue membrane cell extracts (15 µg total protein) fr...
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