Purified anti-KCC2 Antibody (Previously Covance catalog# MMS-5241)

Pricing & Availability
Clone
N1/12 (See other available formats)
Regulatory Status
RUO
Other Names
SLC12A5, rKCC2, K-Cl cotransporter 2, neuronal K-Cl cotransporter, solute carrier family 12, member 5, neuronal-specific K-Cl cotransporter, furosemide-sensitive K-Cl cotransporter, electroneutral potassium-chloride cotransporter 2
Previously
Covance Catalog# MMS-5241
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N1slash12_Purified_KCC2_Antibody_1_021918
Western blot of purified anti-KCC2 antibody (clone LN1/12). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N1slash12_Purified_KCC2_Antibody_1_021918
    Western blot of purified anti-KCC2 antibody (clone LN1/12). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N1slash12_Purified_KCC2_Antibody_2_021918
    IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N1slash12_Purified_KCC2_Antibody_3_021918
    IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N1slash12_Purified_KCC2_Antibody_4_021918
    IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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821701 100 µL £113
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Description

Potassium-chloride cotransporter member 5 (also known as KCC2 and SCL12A5) is responsible for establishing the chloride ion gradient in neurons through the maintenance of low intracellular chloride concentrations.  Mediates synaptic inhibition, protects against excitotoxicity and modulates plasticity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Hartmann A, et al. 2017. PLoS One. 12(6):e0179968. PubMed
RRID
AB_2564853 (BioLegend Cat. No. 821701)

Antigen Details

Structure
Expected MW: 140 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
171373 View all products for this Gene ID
UniProt
View information about KCC2 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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