- Clone
- W17104A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MLKL, HMLKL
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17104A_Purified_MLKL_Antibody_040219 W17104A_Purified_MLKL_Antibody_040219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17104A_Purified_MLKL_Antibody_040219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot analysis of 15µg cell lysates from human PC-3, HaCaT, HeLa (Positive), Daudi (Negative) and Raw264.7 (Mouse) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-MLKL antibody, clone W17104A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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666801 | 25 µg | £70 | ||||
666802 | 100 µg | £174 |
MLKL has a four-helix bundle (4HB) domain at the N-terminal and a C-terminal PsKD connected via a two-helix linker. MLKL is important in the necroptotic signaling pathways. During the process, phosphorylated RIP3 (receptor-interacting protein 3) is recruited and phosphorylates MLKL which can induce the oligomerization of MLKL. Oligomerized MLKL translates to the plasma membrane, destroying the membrane integrity and inducing necroptosis. Knockdown or inhibition of MLKL prohibits necrosis. High expression of MLKL and RIP3 are reported in inflammatory bowel disease. MLKL is also involved in endosomal trafficking and extracellular vesical generation, which is necroptosis independent.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human recombinant MLKL Protein (1-193 a.a.) expressed in E. Coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.5 µg per ml (1:200 - 1:2000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2801124 (BioLegend Cat. No. 666801)
AB_2801125 (BioLegend Cat. No. 666802)
Antigen Details
- Distribution
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Cytoplasm
- Function
- Necroptosis
- Interaction
- RIP3
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Gene ID
- 197259 View all products for this Gene ID
- UniProt
- View information about MLKL on UniProt.org
Related FAQs
Other Formats
View All MLKL Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MLKL | W17104A | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-MLKL
Western blot analysis of 15µg cell lysates from human PC-3, ...
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