- Clone
- 55R-286 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![55R-286_Purified_083107 55R-286_Purified_083107](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/55R-286_Purified_083107.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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NIH/3T3 cell line stained with purified 55R-286, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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113001 | 50 µg | £57 |
CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- E. coli-expressed extracellular domain of the mouse TNFRI protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, ELISA Capture, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. -
Application References
(PubMed link indicates BioLegend citation) -
- Pinckard, J.K., et al. 1997. J. Biol Chem. 272:10784. (IP)
- Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)
- Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC)
- Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC)
- Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912.
- Product Citations
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- RRID
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AB_313530 (BioLegend Cat. No. 113001)
Antigen Details
- Structure
- TNFR superfamily, 55 kD
- Distribution
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Variety of cell types at low levels
- Function
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- Ligand/Receptor
- TNF-α, LT-α (TNF-β)
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798. - Gene ID
- 21937 View all products for this Gene ID
- UniProt
- View information about CD120a on UniProt.org
Related FAQs
Other Formats
View All CD120a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD120a (TNF R Type I/p55) | 55R-286 | FC |
Purified anti-mouse CD120a (TNF R Type I/p55) | 55R-286 | FC,IP,ELISA Capture,WB |
APC anti-mouse CD120a (TNF R Type I/p55) | 55R-286 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD120a (TNF R Type I/p55)
NIH/3T3 cells stained with 55R-286 PE -
Purified anti-mouse CD120a (TNF R Type I/p55)
NIH/3T3 cell line stained with purified 55R-286, followed by... -
APC anti-mouse CD120a (TNF R Type I/p55)
Mouse fibroblast NIH/3T3 cells stained with 55R-286 APC
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