- Clone
- P82H9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P82H9_PURE_Myelin_Basic_Antibody_1_092017 P82H9_PURE_Myelin_Basic_Antibody_1_092017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P82H9_PURE_Myeline_Basic_Antibody_1_092017.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of anti-Myelin Basic Protein antibody (clone P82H9) and isotype-matched IgG1 control. Blots were incubated with anti-MBP at 1 µg/ml (left) or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20 µg rat brain lysates: 20 µg. -
IHC staining of anti-MBP antibody (clone P82H9) on formalin-fixed paraffin-embedded human brain. The tissue was incubated with the primary antibody anti-MBP (left) or mouse IgG1 (right) at 5 µg/mL for 24 hours at 4°C. DAB was used for detection followed by hematoxylin counterstaining.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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850901 | 25 µg | £77 | ||||
850902 | 100 µg | £189 |
Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant MBP protein fragment corresponding to amino acid residues 1-197
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For Immunohistochemistry of paraffin-embedded tissues, the suggested use of this reagent is 1.0 - 5.0 µg per ml It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2716122 (BioLegend Cat. No. 850901)
AB_2716123 (BioLegend Cat. No. 850902)
Antigen Details
- Structure
- Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
- Distribution
-
Tissue distribution: Central and peripheral nervous systems
Cellular distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular - Function
- Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 4155 View all products for this Gene ID
- UniProt
- View information about Myelin Basic Protein on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Myelin Basic Protein Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Myelin Basic Protein | P82H9 | WB,IHC-P |
Alexa Fluor® 594 anti-Myelin Basic Protein | P82H9 | IHC-P,SB |
Alexa Fluor® 488 anti-Myelin Basic Protein | P82H9 | IHC-P,3D IHC,SB |
Alexa Fluor® 647 anti-Myelin Basic Protein | P82H9 | IHC-P,SB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Myelin Basic Protein
Western blot of anti-Myelin Basic Protein antibody (clone P8... IHC staining of anti-MBP antibody (clone P82H9) on formalin-... -
Alexa Fluor® 594 anti-Myelin Basic Protein
IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein a... -
Alexa Fluor® 488 anti-Myelin Basic Protein
IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein a... Formalin-fixed, 300 micron-thick mouse brain (dentate gyrus)... -
Alexa Fluor® 647 anti-Myelin Basic Protein
IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 647 anti-Myelin Basic Protein a...
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