- Clone
- Poly19010 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Paired box protein Pax-2, paired box homeotic gene 2, Opdc, optic disc coloboma
- Previously
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Covance Catalog# PRB-276P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_Purified_Pax-2_Antibody_IHC_021315 1_Purified_Pax-2_Antibody_IHC_021315](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Purified_Pax-2_Antibody_IHC_021315.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules. -
Western blot analysis of cell lysates from MCF-7 (low expression negative control) and Daudi (positive control) cells using Pax-2 rabbit primary antibody (Clone Poly19010,1:1000 dilution, 1 µg/ml) and HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control (1:1000 dilution).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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901002 | 25 µL | £61 | ||||
901001 | 200 µL | £254 |
Pax genes are expressed during embryogenesis in a temporally and spatially restricted manner in the developing neural tube. Pax-2 has been identified to be involved in kidney and optic nerve development. It belongs to the Pax group 2, including Pax-5 and Pax-8. Paired box (Pax) proteins are tissue specific transcription factors that contain a paired domain and typically a partial or complete homeodomain.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse, Chicken, Frog, Zebrafish
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This Pax-2 antibody was generated against a recombinant protein containing 22kD of the Pax-2 sequence corresponding to amino acids 188-385.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
WB - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1-2 µg/ml (1:500-1:1000)
• Gel shifts: 1:10
• IHC: 1:100
Tissue Sections: Formalin-fixed, paraffin-embedded tissues, acetone or PFA fixed frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieva-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature
Staining Localization: nuclear - Application Notes
-
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunopurification and DNA band supershift experiments.
*Predicted MW = 44 kD
Positive Control Tissue: normal kidney or renal adenocarcinoma
This antibody recognizes Pax-2A and Pax-2B (two products of the Pax-2 gene). When linked to agarose beads, it has been used to immunoprecipitate DNA sequences recognized by Pax-2.
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Hammes A, et al. 2001. Cell. 106:319.
- Phelps DE, Dressler GR. 1996. J Biol Chem. 271:7978.
- Rothenpieler UW, Dressler GR. 1993. Development. 119:711
- Dressler GR, et al. 1993. Nature. 362:65
- Dressler GR, Douglass EC. 1992. Proc Natl Acad Sci USA. 89:1179
- Puschel AW, et al. 1992. Mech Dev. 38:197
- Riley BB, et al. 1999. Development. 126:5669. (IHC)
- Product Citations
-
- RRID
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AB_2734656 (BioLegend Cat. No. 901002)
AB_2565001 (BioLegend Cat. No. 901001)
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 5076 View all products for this Gene ID
- UniProt
- View information about Pax-2 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Pax-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Pax-2 | Poly19010 | IHC-P,WB,IP |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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