- Clone
- HWA4C4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Polyubiquitin-C, Polyubiquitin-C precursor, HMG20, Ub-K63, UbK63
- Isotype
- Mouse IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HWA4C4_PURE_Polyubiquitin_Antibody_1_101019 HWA4C4_PURE_Polyubiquitin_Antibody_1_101019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HWA4C4_PURE_Polyubiquitin_Antibody_1_101019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Polyubiquitin (K63-linkage) antibody (clone HWA4C4). Lane 1: Molecular weight marker; Lane 2: 30 µg of HepG2 cell lysate; Lane 3: 30 µg of NIH 3T3 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
Western blot of purified anti-Polyubiquitin (K63-linkage) antibody (clone HWA4C4). Lane 1: Molecular weight marker; Lane 2: 1 µg of Human Poly-Ub WT Chains (1-7) K63 linked protein. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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932201 | 25 µg | £70 | ||||
932202 | 100 µg | £197 |
Polymeric or monomeric Ubiquitin exists free (unanchored) or covalently attached to its ligand. Ubiquitin is conjugated to its target via an isopeptide bond either as a monomer or as one of two polymeric forms. Ubiquitin monomers in one type of polymer are covalently linked via various Lys residues (polyubiquitin). The other type of ubiquitin polymer involves monomers that are linked linearly via an initiator Met (linear polyubiquitin). Polyubiquitin’s functions depend on which Lys residue is involved in cross-linking the protein. K63-linked polyubiquitin chains exhibit nonproteolytic function in vivo which includes kinase/phosphatase activation, protein trafficking, endocytosis, DNA-damage responses, as well as in signaling processes which lead to the activation of the transcription factor NF-κ-B.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide containing the ubiquitin K-63 isopeptide motif.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-F, ICC, IP - Reported in the literature, not verified in house, not verified in-house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone HWA4C4 detects Polyubiquitin chains formed by the linkage with Lys63 residue.
It does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species. -
Application References
(PubMed link indicates BioLegend citation) -
- Dammer E, et al. 2011. J. Biol. Chem. 286:10457. (IHC-F, ICC, WB)
- Lin S, et al. 2013. J. Virology 87:11562. (ICC, IP, WB)
- Karlsson AB, et al. 2014. Mol. Biol. Cell 25:1355. (WB, ICC)
- RRID
-
AB_2566131 (BioLegend Cat. No. 932201)
AB_2566132 (BioLegend Cat. No. 932202)
Antigen Details
- Structure
- 685 amino acids; 77 kD.
- Distribution
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Cytoplasmic, nuclear, and extracellular.
- Function
- Polyubiquitin-C is involved in Poly(A) RNA, protein, and protease binding. The polymeric protein is involved in protein degradation, DNA repair, kinase modification, endocytosis, and cell cycle regulation.
- Interaction
- Numerous targets including CDK-mediated phosphorylation and removal of CDc6, loss of function of SMAD2/3 in cancer, and signaling by NOTCH1 PEST Domain Mutants in cancer.
- Ligand/Receptor
- Poly(A) RNA, protein, and proteases.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Signal Transduction
- Antigen References
-
1. Radici L, et al. 2013. Advances in Bioscience and Biotechnology. 4:1057.
2. Walczak H, et al. 2012. Generation and physiological roles of linear ubiquitin chains. BMC Biol. 10:23. - Gene ID
- 7316 View all products for this Gene ID
- UniProt
- View information about Polyubiquitin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Polyubiquitin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Polyubiquitin (K63-linkage) | HWA4C4 | WB,IHC-F,ICC,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Polyubiquitin (K63-linkage)
Western blot of purified anti-Polyubiquitin (K63-linkage) an... Western blot of purified anti-Polyubiquitin (K63-linkage) an...
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