- Clone
- P87F12H6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PPM1A, Protein Phosphatase 1A, Protein Phosphatase 2C, Alpha isoform, Protein Phosphatase, Mg2+/Mn2+ Dependent 1A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P87F12H6_PURE_PP2C-alpha_Antibody_052019.png P87F12H6_PURE_PP2C-alpha_Antibody_052019.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P87F12H6_PURE_PP2C-alpha_Antibody_052019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-PP2C-α antibody, clone P87F12H6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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617401 | 25 µg | £70 | ||||
617402 | 100 µg | £174 |
Protein Phosphatase 2Cα (PP2C-α) is a ubiquitously expressed magnesium- and manganese-dependent enzyme with a broad specificity of target substrates. It functions by dephosphorylating serine/threonine residues on its target proteins, and has been shown to regulate SMAD, PP2C-α, MAPK, p38 and JNK kinase signaling pathways. PP2C-α deficiency is associated with pancreatic and prostate cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial recombinant human PPC2-α protein corresponding to amino acid residues 202-382.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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When tested for western blot application, this clone produced a non-specific band of roughly ~48 kD in certain cell lines.
- RRID
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AB_2810669 (BioLegend Cat. No. 617401)
AB_2810670 (BioLegend Cat. No. 617402)
Antigen Details
- Structure
- PP2C-α is a 382 amino acid protein with a predicted molecular weight of 42.4 kD.
- Distribution
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Cytosolic distribution/ubiquitously expressed.
- Biology Area
- DNA Repair/Replication, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
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- Schilling SH, et al. 2006. Cell. 125:838.
- Lin X, et al. 2006. Cell. 125:915.
- Chau JF, et al. 2012. Nat. Commun. 3:836.
- Gene ID
- 5494 View all products for this Gene ID
- UniProt
- View information about PP2C-alpha on UniProt.org
Other Formats
View All PP2C-α Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PP2C-α | P87F12H6 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-PP2C-α
Whole cell extracts (15 µg protein) from the indicated cell ...
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