- Clone
- W18011A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TERF2IP, TERF2 Interacting Protein, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, Repressor/activator protein 1 homolog, Dopamine receptor-interacting protein 5, DRIP5
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18011A_PURE_RAP1_Antibody_1_072720 W18011A_PURE_RAP1_Antibody_1_072720](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18011A_PURE_RAP1_Antibody_1_072720.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RAP1 antibody (clone W18011A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Whole cell extracts (15 µg total protein) from Raji lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-RAP1 antibody (clone W18011A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Whole cell extracts (250 µg total protein) prepared from Raji cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2b, κ isotype control antibody (Cat. No. 400602) (panel A) or purified anti-RAP1 antibody (clone W18011A) (panel B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of RAP1. Lane M: Molecular weight marker. -
Raji cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5 µg/mL (1:100 dilution) of either purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) (panel A) or purified anti-RAP1 antibody (clone W18011A) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL (1:250 dilution). Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
938301 | 25 µg | £70 | ||||
938302 | 100 µg | £174 |
Repressor/activator protein 1 (RAP1), also called telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP), is one of six subunits of the shelterin complex, which regulates telomerase activity and protects telomeric DNA through the generation of t-loops. RAP1 forms a heterodimer with shelterin subunit TRF2 and regulates telomere length, recombination, and transcription. This complex also functions in the repression of homology-directed repair (HDR) and prevents telomere deletions. In the cytoplasm, RAP1 functions as an inhibitor kinase (IKK) adaptor and modulator of the nuclear factor- κB (NF-κB) pathway as well as an activator of NF-κB transcriptional activity. TERF2IP S205 phosphorylation has been implicated in the regulation of activation, apoptosis, and senescence of endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human RAP1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not recognize mouse RAP1.
ICC testing was conducted on Raji cells fixed with 4% paraformaldehyde and permeabilized with either Triton X-100 or methanol. Both methods were compatible with RAP1 staining. - RRID
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AB_2876760 (BioLegend Cat. No. 938301)
AB_2876760 (BioLegend Cat. No. 938302)
Antigen Details
- Structure
- RAP1 is a 399 amino acid protein with an expected molecular weight of 55 kD.
- Distribution
-
Ubiquitously expressed/Nuclear
- Function
- Transcription, Transcription regulation
- Biology Area
- Cell Biology, DNA Repair/Replication
- Antigen References
-
- Cai Y, et al. 2017. Cell Cycle. 16:1765-1773.
- Kotla S, et al. 2019. JCI Insight. 4(9):124867.
- Rai R, et al. 2015. Nat Commun. 7:10881.
- Zhang Y, et al. 2015. Cell Death Discov. 1:15007.
- Gene ID
- 54386 View all products for this Gene ID
- UniProt
- View information about RAP1 on UniProt.org
Related FAQs
Other Formats
View All RAP1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RAP1 | W18011A | WB,IP,ICC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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