- Clone
- 16G12A08 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 6, IL-4 Stat
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a)16G12A08_PURE_STAT6_Antibody_WB_011618 a)16G12A08_PURE_STAT6_Antibody_WB_011618](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16G12A08_PURE_STAT6_Antibody_WB_011618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total lysates (15 µg protein) from HeLa (lane1), NTERA-2 (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-STAT6 Antibody, clone 16G12A08 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-STAT6 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder. -
Jurkat cells were stained with purified anti-STAT6 (clone 16G12A08) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue). -
Immunoprecipitation of STAT6 from Jurkat cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-STAT6 antibody (clone 16G12A08, lane 4). Western blot was performed using anti-STAT6 antibody (clone 16G12A08). The astrostar indicates immunoglobulin heavy chain and light chain. -
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT6 (clone 16G12A08), or B) equal amount of purified Mouse Ig1, κ isotype control antibody (Cat. No. 401401) or C) competitor’s ChIP-grade purified anti-STAT6 antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
657901 | 25 µg | £81 | ||||
657902 | 100 µg | £182 |
STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Y641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, transclocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human STAT6 recombinant protein (678-847 aa)
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, ChIP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. For ChIP applications, the suggested use of this reagent is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2562691 (BioLegend Cat. No. 657901)
AB_2562691 (BioLegend Cat. No. 657902)
Antigen Details
- Structure
- 847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization. The C-terminal LXXLL motif is required for interaction with NCOA1.
- Distribution
-
Cytoplasm. Translocates to the nucleus when phosphorylated.
- Function
- STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.
- Interaction
- STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene. 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene. 20:6660.
6. Takeda K, et al. 1996. Nature 380:627. - Gene ID
- 6778 View all products for this Gene ID 20852 View all products for this Gene ID
- UniProt
- View information about STAT6 on UniProt.org
Related FAQs
Other Formats
View All STAT6 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-STAT6 | 16G12A08 | WB,ICC,IP,ChIP |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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