- Clone
- A21125D/G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- von Willebrand factor (vWF), F8VWD, VWD, Coagulation factor VIII antibody, Coagulation factor VIII VWF antibody
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A21125DG_PURE_VWF_Antibody_1_011224 A21125DG_PURE_VWF_Antibody_1_011224](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A21125DG_PURE_VWF_Antibody_1_011224.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of Purified anti-VWF (clone A21125D/G) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated with 5 µg/mL of Purified anti-VWF (clone A21125D/G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 40X objective. Scale bar: 50 µm -
HeLa cells (negative cell line, panel A) and HUVEC cells (positive cell line, panel B) were treated with Brefeldin A (Cat. No. 420601) for 4 hours and fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 10 µg/mL of Purified Mouse IgG2b, κ Isotype Control (clone MPC-11) (Cat. No. 400302) (panels, A-, B-) or 10 µg/mL of Purified anti-VWF (clone A21125D/G) (panels A+, B+), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 µm -
HUVEC cells (positive cell line, filled histogram) and HeLa cells (low-expressing cell line, open histogram, solid line) were treated with Brefeldin A (Cat. No. 420601) for 4 hours and fixed/permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-VWF (clone A21125D/G), or Purified Mouse IgG2b, κ Isotype Control (clone MPC-11) (Cat. No. 400302) (open histogram, dashed line).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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628851 | 25 µg | £113 | ||||
628852 | 100 µg | £284 |
Von Willebrand factor (VWF) plays a crucial role in blood clotting by assisting platelets in forming clots at the site of blood vessel injury. Acting as a bridge between platelets and the damaged blood vessel walls, VWF facilitates platelet adhesion and aggregation, which are essential steps in the clotting process. Moreover, VWF also stabilizes factor VIII, a key protein in the blood clotting cascade, preventing its degradation and enhancing its availability for clot formation. Mutations or deficiencies in VWF results in defects in platelet adhesion and potentially bleeding disorders such as thrombotic thrombocytopenic purpura, Heyde’s syndrome, and von Willebrand disease. A critical region of VWF is the A2 domain. Contained in this domain is the cleavage site for the ADAMTS13 protease which inactivates VWF.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant portion of human VWF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
ICC, ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody (clone A21125D/G) is not suitable for use in western blot.
This antibody (clone A21125D/G) is a pool of two monoclonal antibodies, A21125D and A21125G.
For intracellular flow cytometry (ICFC), fixation/permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401) is recommended. - RRID
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AB_3097540 (BioLegend Cat. No. 628851)
AB_3097540 (BioLegend Cat. No. 628852)
Antigen Details
- Structure
- VWF is a 2,813 amino acid protein with a predicted molecular weight of 309 kD.
- Distribution
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ECM, secreted, cytoplasmic
- Function
- Structural protein, multimeric
- Interaction
- Factor VIII (F8), ADAMTS13, GPIb receptor, heparin, collagen, platelet integrin αIIbβ3
- Cell Type
- Platelets
- Antigen References
-
- Randi AM, et al. 2018. Blood. 132:132-40.
- Xiang Y & Hwa J. 2016. Curr Opin Hematol. 23:288-93.
- Chen J & Chung DW. 2018. Blood. 132:141-147.
- Lynch CJ, et al. 2014. Blood. 123:2585-92.
- Gene ID
- 7450 View all products for this Gene ID
- UniProt
- View information about VWF on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All VWF Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-VWF | A21125D/G | IHC-P,ICC,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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