Tag-it Violet™ Proliferation and Cell Tracking Dye

Pricing & Availability
Regulatory Status
RUO
Other Names
Tag-It Violet Proliferation Cell Tracking Dye
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Product Citations
publications
Tag-it_Violet_Cell_Proliferation_Cell_Tracking_Dye_1_FC_122115
Human peripheral blood mononuclear cells were stained with Tag-it Violet™ Proliferation and Cell Tracking dye, and then stimulated with PHA for four days. On day four, cells were harvested, stained with CD3-PE and the Tag-it Violet™ signal was analyzed by flow cytometry.
  • Tag-it_Violet_Cell_Proliferation_Cell_Tracking_Dye_1_FC_122115
    Human peripheral blood mononuclear cells were stained with Tag-it Violet™ Proliferation and Cell Tracking dye, and then stimulated with PHA for four days. On day four, cells were harvested, stained with CD3-PE and the Tag-it Violet™ signal was analyzed by flow cytometry.
  • Tag-it_Violet_Cell_Proliferation_Cell_Tracking_Dye_2_ICC_020421
    Mouse spleen 72 hours after adoptive transfer of Tag-it Violet™-labeled splenocytes (purple). Nucleated cells are stained using 25 µM DRAQ5™ (red). Image was captured at a 40X magnification.
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425101 1 kit £182
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Description

Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell tracking and for proliferation assays. Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ then covalently attaches to intracellular proteins enabling its long-term retention.

Tag-it Violet™ is excited with a fluorescence wavelength of 395 nm, and emits at 455 nm. Cells labeled wtih Tag-it Violet™ can be analyzed by flow cytometry in instruments equipped with a violet laser, or visualized by fluorescence microscopy using a DAPI filter.

Product Details
Technical Data Sheet (pdf)

Product Details

Preparation
The Tag-it Violet™ Proliferation and Cell Tracking Dye is composed of lyophilized Tag-it Violet™ and anhydrous DMSO. For reconstitution, bring the kit to room temperature, add 50 µl of DMSO to one vial of Tag-it Violet™ dye until fully dissolved.
Storage & Handling
The Tag-it Violet™ Proliferation and Cell Tracking Dye should be stored at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Tag-it Violet™, use immediately, or store at -20°C in a dry place and protected ifrom light, preferably in a dessicator or in a container with dessicant for no more than one month.
Application

FC - Quality tested
ICC - Verified

Recommended Usage

This lot has been tested by flow cytometry for in vitro cell proliferation. It can be used at concentrations ranging from 1 - 20 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.

Application Notes

The molecular weight of Tag-it Violet™ Proliferation and Cell Tracking Dye is 489 kD. The maximum excitation and emission wavelengths of Tag-it Violet™ Proliferation and Cell Tracking Dye are 395 nm and 455 nm, respectively. Each 122.25 µg of Tag-it Violet™ Proliferation and Cell Tracking Dye may be reconstituted with 50 µl of anhydrous DMSO to yield a stock concentration of 5 mM.

Materials Provided:
5 vials x 122.25 µg Tag-it Violet™
500 µl anhydrous DMSO

Tag it-Violet™ Labeling Procedure:
1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
2. Prepare stock solution by reconstituting 1 vial of lyophilized Tag-it Violet™ dye in 50 µl of anhydrous DMSO to make a 5 mM solution.
3. Prepare a 5 µM working solution by diluting 1 µL of 5 mM Tag-it Violet™ stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration).
4. Spin down and resuspend cells at 10-100 x 106 cells/mL in the Tag-it Violet™ working solution.
5. Incubate cells for 20 minutes at room temperature or at 37°C, and keep protected from light.
6. Quench the staining by adding five times the original staining volume of cell culture medium containing 10% FBS.
7. Pellet cells and resuspend in pre-warmed cell culture medium. 
8. Incubate cells for 10 minutes.
9. After incubation, Tag-it Violet™ labeled cells are ready for downstream applications or analysis.

Product Citations
  1. Alsavaf MB, et al. 2022. Br J Haematol. 197:e88. PubMed
  2. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  3. Zaiatz-Bittencourt V, et al. 2023. Sci Rep. 13:2715. PubMed
  4. Sheng YR, et al. 2022. Cell Mol Life Sci. 79:173. PubMed
  5. Mehus AA, et al. 2023. Int J Mol Sci. 24:. PubMed
  6. Olivieri D, et al. 2021. EMBO J. 40:e106818. PubMed
  7. Santelli A, et al. 2019. J Am Heart Assoc. 8:e012584. PubMed
  8. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  9. Kumar V, et al. 2022. JACC Basic Transl Sci. 7:1038. PubMed
  10. Denham EM, et al. 2019. PLoS Biol. 17:e3000549. PubMed
  11. Guo X, et al. 2021. iScience. 24:103295. PubMed

Antigen Details

Structure
489 kD.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Gene ID
NA

Related FAQs

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Go To Top Version: 3    Revision Date: 02/04/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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