- Clone
- 2C9.G2 (HMβ3-1) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β3 integrin, Integrin β3 chain, gpIIIa
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2C9dotG2_LEAF_090707.jpg 2C9dotG2_LEAF_090707.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2C9dotG2_LEAF_090707.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells stained with Ultra-LEAF™ purified 2C9.G2, followed by anti-Armenian hamster IgG FITC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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104325 | 100 µg | £121 | ||||
104326 | 1 mg | £464 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv/β3 integrin or vitronectin receptor to CD31 (PECAM-1).
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
Block, Activ, IHC-F - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).
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Application References
(PubMed link indicates BioLegend citation) -
- Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
- Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
- Ashkar S, et al. 2000. Science 287:860. (Block)
- Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
- Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
- Carlson TR, et al. 2008.135:2193. PubMed
- Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
- RRID
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AB_2832322 (BioLegend Cat. No. 104325)
AB_2832323 (BioLegend Cat. No. 104326)
Antigen Details
- Structure
- Integrin family, 110 kD
- Distribution
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Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
- Function
- Attachment to matrix
- Ligand/Receptor
- Fibrinogen, von Willebrand factor, fibronectin
- Cell Type
- B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell. 65:359.
3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864. - Gene ID
- 16416 View all products for this Gene ID 29302 View all products for this Gene ID
- UniProt
- View information about CD61 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD61 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Biotin anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
FITC anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
PE anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
Purified anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC,IHC |
Alexa Fluor® 488 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
Alexa Fluor® 647 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
APC anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
PE/Cyanine7 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
PerCP/Cyanine5.5 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
PE/Dazzle™ 594 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC |
TotalSeq™-C0910 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | PG |
Ultra-LEAF™ Purified anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | FC,Block,Activ,IHC |
TotalSeq™-A0910 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | PG |
TotalSeq™-B0910 anti-mouse/rat CD61 | 2C9.G2 (HMβ3-1) | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with biotinylated 2C... -
FITC anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with HMβ3-1 FITC -
PE anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with 2C9.G2 PE -
Purified anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with purified 2C9.G2... -
Alexa Fluor® 488 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with 2C9.G2 Alexa Fl... -
Alexa Fluor® 647 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with 2C9.G2 Alexa Fl... -
APC anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells were stained with CD61 (clon... -
PE/Cyanine7 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells were stained with CD61 (clon... -
PerCP/Cyanine5.5 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells were stained with CD61 (clon... -
PE/Dazzle™ 594 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells were stained with CD61 (clon... -
TotalSeq™-C0910 anti-mouse/rat CD61
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Ultra-LEAF™ Purified anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with Ultra-LEAF™ pur... -
TotalSeq™-A0910 anti-mouse/rat CD61
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TotalSeq™-B0910 anti-mouse/rat CD61
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