- Clone
- RMG2b-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2b
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMG2b-1_A488_IgG2b_Antibody_1_062620 RMG2b-1_A488_IgG2b_Antibody_1_062620](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMG2b-1_A488_IgG2b_Antibody_1_062620.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) (filled histogram) or purified mouse IgG2b (clone MPC-11) isotype control (open histogram) followed by anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488. -
C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488 (green) for 1 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with the MetaMorph® software.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
406717 | 25 µg | £57 | ||||
406718 | 100 µg | £121 |
The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Product Citations
- RRID
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AB_2876732 (BioLegend Cat. No. 406717)
AB_2876732 (BioLegend Cat. No. 406718)
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