- Clone
- RIKO-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Decay accelerating factor (DAF)
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RIKO-3_A647_121508.jpg RIKO-3_A647_121508.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RIKO-3_A647_121508.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes stained with RA3-6B2 (B220) PE and Riko-3 Alexa Fluor® 647
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
131805 | 25 µg | £101 | ||||
131806 | 100 µg | £238 |
CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Cao Q, et al. 2012. Am J Physiol Cell Physiol. 302:1731. PubMed.
- Product Citations
- RRID
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AB_1279263 (BioLegend Cat. No. 131805)
AB_1279261 (BioLegend Cat. No. 131806)
Antigen Details
- Structure
- 60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
- Distribution
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Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
- Function
- Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
- Cell Type
- B cells, Erythrocytes, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
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1. Miwa T et al. 2001. Immunology 104:207
2. Lin FY et al. 2001 Immunology 104:215 - Gene ID
- 13136 View all products for this Gene ID
- UniProt
- View information about CD55 on UniProt.org
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