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Alexa Fluor® 700 anti-human CD16 Antibody

Pricing & Availability
Clone
3G8 (See other available formats)
Regulatory Status
RUO
Workshop
V NK80
Other Names
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
3G8_Alx700_022008
Human peripheral blood lymphocytes stained with 3G8 Alexa fluor® 700
  • 3G8_Alx700_022008
    Human peripheral blood lymphocytes stained with 3G8 Alexa fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
302025 25 µg £85
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302026 100 µg £184
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Lusty E, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.025. PubMed
  2. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  3. de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
  4. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  5. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  6. Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed
  7. Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
  8. Vaccari M, et al. 2013. J Virol. 87:3526. PubMed
  9. Chalan P, et al. 2015. PLoS One. 10: 0132436. PubMed
  10. Pate K, et al. 2015. J Infect Dis. 212: 1387 - 1396. PubMed
  11. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  12. Chowdhury A, et al. 2015. J Virol. 89: 8677-8686. PubMed
  13. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  14. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  15. Koutsakos M, et al. 2021. Cell Reports Medicine. 2(3):100208. PubMed
  16. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  17. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  18. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  19. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  20. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  21. Carre C, et al. 2021. iScience. 24:102970. PubMed
  22. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  23. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  24. Veenhuis RT, et al. 2021. JCI Insight. 6:. PubMed
  25. Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed
  26. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  27. Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
  28. Georg P, et al. 2022. Cell. 185:493. PubMed
  29. NULL, et al. 2022. Cell. 185:916. PubMed
  30. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  31. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
  32. Connors J, et al. 2022. Geroscience. :. PubMed
  33. Diny NL, et al. 2022. J Exp Med. 219: . PubMed
  34. Lee HS, et al. 2023. Biol Pharm Bull. 46:542. PubMed
RRID
AB_493748 (BioLegend Cat. No. 302025)
AB_2278418 (BioLegend Cat. No. 302026)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Go To Top Version: 3    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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