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Alexa Fluor® 700 anti-human CD4 Antibody

Pricing & Availability
Clone
SK3 (See other available formats)
Regulatory Status
RUO
Other Names
T4, Leu3a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK3_AF700_CD4_Antibody_FC_012914.jpg
Human peripheral blood lymphocytes were stained with CD4 (clone SK3) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 (open histogram).
  • SK3_AF700_CD4_Antibody_FC_012914.jpg
    Human peripheral blood lymphocytes were stained with CD4 (clone SK3) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 (open histogram).
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
344621 25 tests £72
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344622 100 tests £163
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Evans RL, et al. 1981. Immunol. 78:544
  2. Arno A et al. 1999. J. Infect. Dis. 180:56
  3. Muech M, et al. 1997. Blood 89:1364
  4. Wang L, et al. 2012. Cytometry A. 81:567. PubMed
Product Citations
  1. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  3. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  4. Martin AR, et al. 2020. EBioMedicine. 62:103102. PubMed
  5. Litchfield K, et al. 2021. Cell. 184(3):596-614.e14. PubMed
  6. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  7. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  8. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  9. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  10. Turner JS, et al. 2020. Nature. 586:127. PubMed
  11. Lorvik KB, et al. 2021. Front Immunol. 12:744155. PubMed
  12. Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed
  13. Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed
  14. Morita R, et al. 2023. iScience. 106685:26. PubMed
RRID
AB_2563149 (BioLegend Cat. No. 344621)
AB_2563150 (BioLegend Cat. No. 344622)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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