- Clone
- TY/11.8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ecto-5'-nucleotidase
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TY-11dot8_A700_CD73_Antibody_022519 TY-11dot8_A700_CD73_Antibody_022519](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TY-11dot8_A700_CD73_Antibody_022519.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were first treated with TruStain FcX™ (anti-mouse CD16/32) antibody and True-Stain Monocyte Blocker™. Cells were then stained with CD3ε PE and CD73 (clone TY/11.8) Alexa Fluor® 700 (left) or rat IgG1, κ Alexa Fluor® 700 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
127229 | 25 µg | £105 | ||||
127230 | 100 µg | £201 |
CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO cells transfected with mouse CD73
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
- Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
- Product Citations
- RRID
-
AB_2800624 (BioLegend Cat. No. 127229)
AB_2800625 (BioLegend Cat. No. 127230)
Antigen Details
- Structure
- 69 kD GPI-anchored surface protein
- Distribution
-
Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
- Function
- A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
- Ligand/Receptor
- Nucleoside 5' monophosphate
- Cell Type
- B cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95. - Regulation
- Expression is regulated by the development and maturation of leukocytes
- Gene ID
- 23959 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
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