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Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
Clone
30-H12 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-H12_Alx700_021808
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 700
  • 30-H12_Alx700_021808
    C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
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105319 25 µg £70
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105320 100 µg £157
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  2. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  3. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  4. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  5. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  6. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  7. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  8. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  9. Seedhom M, et al. 2012. J Virol. 86:6010. PubMed
  10. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  11. Monticelli L, et al. 2015. Proc Natl Acad Sci U S A. 112: 10762-10767. PubMed
  12. Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed
  13. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  14. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  15. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  16. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  17. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  18. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  19. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  20. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  21. Goc J, et al. 2021. Cell. 184:5015. PubMed
  22. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  23. Lu P, et al. 2021. Nat Commun. 12:1042. PubMed
  24. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  25. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  26. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  27. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  28. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  29. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  30. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  31. Skladanowska K, et al. 2022. Cell Rep. 41:111490. PubMed
  32. Matter AL, et al. 2022. J Immunol. 209:1880. PubMed
  33. Matsushita Y, et al. 2023. Nat Commun. 14:2383. PubMed
RRID
AB_493724 (BioLegend Cat. No. 105319)
AB_493725 (BioLegend Cat. No. 105320)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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