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Alexa Fluor® 700 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_A700_NK11_Antibody_041918
C57BL/6 splenocytes were stained with CD49b (clone DX5) PE and NK1.1 (clone PK136) Alexa Fluor® 700 (left) or Mouse IgG2a, κ Alexa Fluor® 700 isotype control (right).
  • PK136_A700_NK11_Antibody_041918
    C57BL/6 splenocytes were stained with CD49b (clone DX5) PE and NK1.1 (clone PK136) Alexa Fluor® 700 (left) or Mouse IgG2a, κ Alexa Fluor® 700 isotype control (right).
See Alexa Fluor® 700 spectral data
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108729 25 µg £68
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108730 100 µg £155
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  2. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  3. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  4. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  5. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  6. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  7. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  8. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  9. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  10. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  11. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  12. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  13. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  14. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  15. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  16. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  17. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  18. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  19. Laumonnier Y, et al. 2020. Curr Protoc Immunol. 130:e100. PubMed
  20. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  21. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  22. Roy R, et al. 2022. Elife. 11:. PubMed
  23. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  24. Lee HY, et al. 2021. Curr Protoc. 1:e272. PubMed
  25. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  26. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  27. Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed
RRID
AB_2074426 (BioLegend Cat. No. 108729)
AB_2291262 (BioLegend Cat. No. 108730)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org
Go To Top Version: 4    Revision Date: 04/19/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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