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APC anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_APC_020708
Human colon carcinoma cell line HT29 stained with 9C4 APC
  • 9C4_APC_020708
    Human colon carcinoma cell line HT29 stained with 9C4 APC
See APC spectral data
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324207 25 tests £102
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324208 100 tests £215
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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Menck K, et al. 2017. J Extracell Vesicles. 10.1080/20013078.2017.1340745. PubMed
  2. Apostolou P, Ntanovasilis D, and Papasotiriou I. 2017. J Biol Res (Thessalon). 10.1186/s40709-017-0068-9. PubMed
  3. Chua CW, et al. 2018. Elife. 7. PubMed
  4. Puca L, et al. 2018. Nat Commun. 9:2404. PubMed
  5. Lo PK, et al. 2018. Elife. 7:e31334. PubMed
  6. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  7. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  8. Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
  9. Kojima Y et al. 2017. Cell stem cell. 21(4):517-532 . PubMed
  10. Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
  11. Enge M, et al. 2017. Cell. 171:321. PubMed
  12. Liu L, et al. 2018. Clin Cancer Res. 24:2370. PubMed
  13. Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed
  14. Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed
  15. Mota STS, et al. 2019. Int J Mol Sci. 20. PubMed
  16. Ludwig LS et al. 2019. Cell. 176(6):1325-1339 . PubMed
  17. Fujii M, et al. 2018. Cell Stem Cell. 23:787. PubMed
  18. Wosen JE, et al. 2019. Front Immunol. 1.784722222. PubMed
  19. Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
  20. Mithal A, et al. 2020. Nat Commun. 0.607638889. PubMed
  21. Yan X, et al. 2019. Oncol Rep. 41:1938. PubMed
  22. Balakrishnan A, et al. 2019. Clin Cancer Res. 25:7506. PubMed
  23. Yamashiro C, et al. 2020. Nature Protocols. 362(6412):356-360. PubMed
  24. Tang C, et al. 2020. Stem Cell Research. 39:101511.. PubMed
  25. Yamaguchi N, et al. 2020. eLife. 8:e52135.. PubMed
  26. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  27. Skrbo N, et al. 2014. PLoS One. 9:113278. PubMed
  28. Nelson L, et al. 2015. Sci Rep. 5: 17455. PubMed
  29. Haraguchi T, et al. 2016. Sci Rep. 6:21117. PubMed
  30. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  31. Yoshida K, et al. 2020. Nature. 578:266. PubMed
  32. Mou H, et al. 2021. Cell Reports. 35(3):109011. PubMed
  33. Mulay A, et al. 2021. Cell Reports. 35(5):109055. PubMed
  34. Hornburg M, et al. 2021. Cancer Cell. . PubMed
  35. Grant RA, et al. 2021. Nature. 590(7847):635-641. PubMed
  36. Monterosso ME, et al. 2021. Sci Rep. 5118:11. PubMed
  37. Murase Y, et al. 2020. EMBO J. 39:e104929. PubMed
  38. Yuan W, et al. 2021. Int J Biol Sci. 17:527. PubMed
  39. Koh SB, et al. 2021. NPJ Precis Oncol. 5:85. PubMed
  40. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  41. Xiang X, et al. 2022. Nat Commun. 13:463. PubMed
  42. Marquez-Curtis LA, et al. 2022. PLoS One. 17:e0263005. PubMed
  43. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  44. Yu XX, et al. 2021. Cell Res. 31:886. PubMed
  45. Nian Z, et al. 2021. Clin Cancer Res. 27:6026. PubMed
  46. Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed
  47. Yamashiro C, et al. 2020. Nat Protoc. 15:1560. PubMed
  48. Go YH, et al. 2022. Cancers (Basel). 14:. PubMed
  49. Maughan EF, et al. 2022. iScience. 25:105409. PubMed
  50. Kadur Lakshminarasimha Murthy P, et al. 2022. Dev Cell. 57:310. PubMed
  51. Wang Y, et al. 2022. Cancer Res. 82:3903. PubMed
  52. Cabello P, et al. 2023. Cancers (Basel). 15:. PubMed
  53. Beckinger S, et al. 2023. Front Oncol. 13:1160824. PubMed
  54. Golubovskaya V, et al. 2023. Cancers (Basel). 15:. PubMed
RRID
AB_756081 (BioLegend Cat. No. 324207)
AB_756082 (BioLegend Cat. No. 324208)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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