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APC anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
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Product Citations
publications
HI100_APC_062707
Human peripheral blood lymphocytes stained with HI100 APC
  • HI100_APC_062707
    Human peripheral blood lymphocytes stained with HI100 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
304111 25 tests £38
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304112 100 tests £91
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304150 100 µg £94
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Hazeldine J, et al. 2017. PLoS Med. 10.1371/journal.pmed.1002338. PubMed
  2. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  3. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  4. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  5. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed
  6. Mesquitta WT, et al. 2019. Sci Rep. 4.973611111. PubMed
  7. Christiansen M, et al. 2020. Front Immunol. 10:3022. PubMed
  8. Thomas R, et al. 2020. Cancer Immunol Immunother. 69:449. PubMed
  9. Ribot J, et al. 2014. J Immunol. 192:2237. PubMed
  10. Li Z, et al. 2014. PLoS One. 9:106064. PubMed
  11. Kim Y, et al. 2015. Blood. 125:1107. PubMed
  12. Qin H, et al. 2015. Blood. 126: 629 - 639. PubMed
  13. Sinnott B, et al. 2016. J Immunol. 197: 68 - 77. PubMed
  14. Tomalka J, Jesus T, Ramakrishnan P 2016. J Leukoc Biol. 100: 111 - 120. PubMed
  15. Seumois G, et al. 2016. J Immunol. 197: 655 - 664. PubMed
  16. Locke F, et al. 2017. Mol Ther. 25(1):285-295. PubMed
  17. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  18. Wang J, et al. 2020. Cell. 183:1264. PubMed
  19. Stunnenberg M, et al. 2020. Viruses. 12:00. PubMed
  20. Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed
  21. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  22. Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed
  23. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  24. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  25. Aschmoneit N, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  27. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  28. Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
  29. Hebbar N, et al. 2022. Nat Commun. 13:587. PubMed
  30. Wu K, et al. 2021. Aging (Albany NY). 13:. PubMed
  31. Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
  32. Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
  33. Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed
  34. Du L, et al. 2021. Front Mol Biosci. 8:675179. PubMed
  35. Xiao C, et al. 2022. iScience. 25:103934. PubMed
  36. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  37. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  38. Yazdani S, et al. 2022. Nat Commun. 13:6733. PubMed
  39. Cox MJ, et al. 2022. Leukemia. 36:1635. PubMed
  40. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  41. Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed
  42. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  43. Yen M, et al. 2022. Cell. 185:1414. PubMed
RRID
AB_314415 (BioLegend Cat. No. 304111)
AB_314416 (BioLegend Cat. No. 304112)
AB_2564158 (BioLegend Cat. No. 304150)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org
Go To Top Version: 2    Revision Date: 07/25/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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