- Clone
- BB7.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC-class I
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BB7.2_APC_HLA-A2_Antibody_FC_1_092812 BB7.2_APC_HLA-A2_Antibody_FC_1_092812](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BB7.2_APC_HLA-A2_Antibody_FC_1_092812.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) APC (filled histograms) or mouse IgG2b APC isotype control (open histograms). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
343307 | 25 tests | £86 | ||||
343308 | 100 tests | £223 |
HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Papain solubilized HLA-A2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.
Additional reported applications (for the relevant formats) include: immunoprecipitation3. -
Application References
(PubMed link indicates BioLegend citation) -
- Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
- Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
- Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
- Product Citations
- RRID
-
AB_2561566 (BioLegend Cat. No. 343307)
AB_2561567 (BioLegend Cat. No. 343308)
Antigen Details
- Structure
- Ig superfamily
- Distribution
-
A*02 individuals leukocytes
- Function
- Antigen presentation
- Ligand/Receptor
- CD8, CD3/TCR
- Cell Type
- Leukocytes
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Gene ID
- 3105 View all products for this Gene ID
- UniProt
- View information about HLA-A2 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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