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APC anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1_APC_CD80_Antibody_FC_091014.jpg
LPS-stimulated (3 days) BALB/c splenocytes were stained with CD80 (clone 16-10A1) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
  • 16-10A1_APC_CD80_Antibody_FC_091014.jpg
    LPS-stimulated (3 days) BALB/c splenocytes were stained with CD80 (clone 16-10A1) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Check Availability Save
104713 25 µg £61
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104714 100 µg £181
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  2. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  3. Peng KT, et al. 2019. Int J Nanomedicine. 14:469. PubMed
  4. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  5. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  6. Xue RY, et al. 2019. Front Immunol. 10:1372. PubMed
  7. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  8. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  9. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  10. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  11. Mino T, et al. 2019. Nucleic Acids Res. 47:8838. PubMed
  12. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  13. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  14. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  15. Onodera T, et al. 2012. Proc Natl Acad Sci U S A. 109:2485. PubMed
  16. Assi H, et al. 2014. PLoS One. 9:96318. PubMed
  17. Škrnjug I, et al. 2014. PLoS One. 9:110150. PubMed
  18. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  19. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  20. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  21. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  22. Chou S, et al. 2016. J Control Release. 244:14-23. PubMed
  23. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  24. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  25. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  26. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  27. Angulo G, et al. 2021. eLife. 10:00. PubMed
  28. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  29. Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed
  30. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  31. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  32. Guo S, et al. 2021. Chronic Dis Transl Med. 7:254. PubMed
  33. Guan X, et al. 2022. Nat Commun. 13:2834. PubMed
  34. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  35. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  36. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  37. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  38. Wang X, et al. 2022. J Biol Chem. 298:101561. PubMed
  39. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  40. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  41. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  42. Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed
  43. Mao L, et al. 2022. Front Immunol. 13:833418. PubMed
  44. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  45. Yao RQ, et al. 2022. Theranostics. 12:4606. PubMed
  46. Català C, et al. 2022. iScience. 25:105078. PubMed
  47. Diao L, et al. 2022. iScience. 25:105511. PubMed
  48. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  49. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  50. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  51. Yu J, et al. 2022. Nat Commun. 13:7903. PubMed
  52. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  53. An S, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_313134 (BioLegend Cat. No. 104713)
AB_313135 (BioLegend Cat. No. 104714)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org
Go To Top Version: 4    Revision Date: 09/11/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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