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APC anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_APC_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 APC
  • RA3-6B2_APC_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 APC
See APC spectral data
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103211 25 µg £20
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103212 100 µg £61
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45R antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  2. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  3. Prajeeth C,et al. 2017. J Neuroinflammation.. 10.1186/s12974-017-0978-3. PubMed
  4. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  5. Neumann AM et al. 2017. PloS one. 12(11):e0187330 . PubMed
  6. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  7. Liu S, et al. 2018. Front Immunol. 29:208. PubMed
  8. Kim SK, et al. 2018. Genes Dev. 32:849. PubMed
  9. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  10. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  11. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  12. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  13. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  14. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  15. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  16. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  17. Graf R et al. 2019. Cell reports. 26(5):1098-1103 . PubMed
  18. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  19. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  20. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  21. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  22. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  23. Bussey KA, et al. 2019. J Virol. 93. PubMed
  24. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  25. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  26. Li X, et al. 2018. Immunity. 48:530. PubMed
  27. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  28. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  29. Perper SJ, et al. 2019. J Immunol. 203:58. PubMed
  30. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
  31. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  32. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  33. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  34. Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
  35. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  36. Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed
  37. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  38. Escolano A, et al. 2019. Nature. 570:468. PubMed
  39. Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed
  40. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  41. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  42. Riley JS, et al. 2020. Blood Adv. 4:1102. PubMed
  43. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
  44. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  45. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  46. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  47. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  48. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  49. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
  50. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  51. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  52. Shih F, et al. 2006. J Immunol. 176:3438. PubMed
  53. Cervantes-Barragan L, et al. 2007. Blood . 109:1131. PubMed
  54. Zhang Y, et al. 2008. Mol Cell Biol. 28:1688. PubMed
  55. Hirata Y, et al. 2010. Blood. 115:3930. PubMed
  56. Fahey L, et al. 2011. J Exp Med. 208:987. PubMed
  57. Recher M, et al. 2012. Blood. 119:2819. PubMed
  58. Sun S, et al. 2013. J Immunol. 190:6209. PubMed
  59. Davidson M, et al. 2013. PLoS One. 8:76258. PubMed
  60. Takaki H, et al. 2013. J Immunol. 191:4740. PubMed
  61. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  62. Clemens E, et al. 2015. J Immunol. 194:898. PubMed
  63. Puget N, et al. 2015. Mol Cell Biol. 35:529. PubMed
  64. Feldman S, et al. 2015. J Immunol. 194:2380. PubMed
  65. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  66. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  67. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  68. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  69. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  70. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  71. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  72. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  73. Braikia F, et al. 2015. Mol Cell Biol. 35: 3370 - 3380. PubMed
  74. Yammani R, et al. 2015. J Immunol. 193: 5434-5443. PubMed
  75. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  76. Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed
  77. Saintamand A, et al. 2016. Nat Commun. 7:10730. PubMed
  78. Li Y, et al. 2016. Nat Commun. 7:10836. PubMed
  79. Nocera D, et al. 2016. J Immunol. 196: 2860 - 2869. PubMed
  80. Odeh A Simecka J 2016. PLoS One. 11: 0155648. PubMed
  81. Cooley L, et al. 2016. Sci Rep. 6: 25840. PubMed
  82. Toivonen R, et al. 2016. J Immunol. 196: 4750 - 4759. PubMed
  83. Ma Z, et al. 2016. Clin Cancer Res. 22: 2969 - 2980. PubMed
  84. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  85. Hohmann A, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2115. PubMed
  86. Novkovic M, et al. 2016. PLoS Biol. 14: 1002515. PubMed
  87. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  88. Noir S, et al. 2017. Nucleic Acids Res. 10.1093/nar/gkx203. PubMed
  89. Perfilyeva Y, et al. 2017. Inflammation Research. 10.1007/s00011-017-1052-4. PubMed
  90. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  91. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  92. Li K, et al. 2020. Nat Commun. 4.844444444. PubMed
  93. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  94. Li J, et al. 2020. Elife. 9:00. PubMed
  95. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  96. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  97. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  98. Schaum N, et al. 2020. Nature. 583:596. PubMed
  99. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  100. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  101. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  102. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  103. Tartey S, et al. 2021. Cancer Research. 81(9):2358-2372. PubMed
  104. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  105. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  106. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  107. Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
  108. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  109. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  110. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  111. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  112. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  113. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  114. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  115. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  116. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  117. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  118. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  119. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  120. Reddi RN, et al. 2021. J Am Chem Soc. 143:4979. PubMed
  121. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  122. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  123. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  124. Man N, et al. 2021. JCI Insight. 6:. PubMed
  125. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  126. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  127. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  128. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  129. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  130. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  131. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  132. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  133. Koide S, et al. 2022. iScience. 25:103603. PubMed
  134. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  135. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  136. Ma K, et al. 2022. iScience. 25:104347. PubMed
  137. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  138. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  139. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  140. Yang Z, et al. 2021. Sci Adv. 7:. PubMed
  141. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  142. He R, et al. 2021. J Immunol. 206:2353. PubMed
  143. Wüthrich M, et al. 2021. MBio. 12:e0201821. PubMed
  144. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  145. Woodring T, et al. 2022. iScience. 25:104934. PubMed
  146. Wang Q, et al. 2020. Wound Repair Regen. 28:105. PubMed
  147. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  148. Li C, et al. 2021. Chem Biol Drug Des. 97:1151. PubMed
  149. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  150. Reddi RN, et al. 2021. J Am Chem Soc. 143:20095. PubMed
  151. Di Martino L, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:901. PubMed
  152. Kohn EM, et al. 2022. J Immunol. 208:1417. PubMed
  153. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  154. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  155. Stone VM, et al. 2022. iScience. 25:105070. PubMed
  156. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  157. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  158. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  159. Erickson JJ, et al. 2022. Nature. 606:769. PubMed
  160. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  161. Roh YJ, et al. 2023. Lab Anim Res. 39:2. PubMed
  162. Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed
  163. Roberts JL, et al. 2022. Bone. 157:116310. PubMed
  164. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  165. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  166. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  167. Sia ZR, et al. 2022. J Virol. 96:e0100622. PubMed
  168. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  169. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  170. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  171. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  172. Wiedmann L, et al. 2023. Nat Commun. 14:2353. PubMed
RRID
AB_312996 (BioLegend Cat. No. 103211)
AB_312997 (BioLegend Cat. No. 103212)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org
Go To Top Version: 2    Revision Date: 04/25/2013

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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