- Clone
- 104D2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![104D2_BV421_062911 104D2_BV421_062911](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/104D2_BV421_062911.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human erythroleukemia cell line (HEL) was stained with CD117 (clone 104D2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
313215 | 25 tests | £140 | ||||
313216 | 100 tests | £261 |
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocytic cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
- RRID
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AB_10896056 (BioLegend Cat. No. 313215)
AB_11148721 (BioLegend Cat. No. 313216)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
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Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. - Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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