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Brilliant Violet 510™ anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5114.15.2_BV510_IAIE_Antibody_FC_091712
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).
  • M5114.15.2_BV510_IAIE_Antibody_FC_091712
    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).
See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability Save
107635 125 µL £121
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107636 50 µg £184
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
µg & µl: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
µg & µl: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
µg & µl: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using µg size, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For flow cytometric staining using µl size, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Eddy W, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1601777. PubMed
  2. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
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  4. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
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  10. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  11. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  12. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  13. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  14. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  15. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  16. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  17. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  18. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  19. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  20. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  21. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  22. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  23. Lammers K, et al. 2015. PLoS One. 10: 0138338. PubMed
  24. Yasuda T, et al. 2016. PLoS One. 11:e0167952. PubMed
  25. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  26. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  27. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  28. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  29. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  30. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  31. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  32. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  33. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  34. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  35. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
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  37. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
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  41. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  42. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  43. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  44. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  45. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  46. Huang YL, et al. 2021. Commun Biol. 4:229. PubMed
  47. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  48. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  49. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  50. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  51. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  52. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  53. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
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  55. Wu X, et al. 2021. Elife. 10:. PubMed
  56. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
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  61. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
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RRID
AB_2561397 (BioLegend Cat. No. 107635)
AB_2734168 (BioLegend Cat. No. 107636)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org
Go To Top Version: 4    Revision Date: 10/30/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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