- Clone
- BC96 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V T-072
- Other Names
- Low affinity IL-2R, IL-2R α chain, Tac, p55
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BC96_BV650_1_052112 BC96_BV650_1_052112](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BC96_BV650_1_052112.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD4 FITC and CD25 (clone BC96) Brilliant Violet 650™ (top) or mouse IgG1, κ Brilliant Violet 650™ isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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302633 | 25 tests | £163 | ||||
302634 | 100 tests | £291 |
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Additional reported applications include: immunocytochemistry3.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
- RRID
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AB_11203536 (BioLegend Cat. No. 302633)
AB_2563807 (BioLegend Cat. No. 302634)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
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Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681. - Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
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