- Clone
- A17020B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S6, 40S ribosomal protein S6
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A17020B_DB-HRP_RPS6-Phospho_Antibody_022522 A17020B_DB-HRP_RPS6-Phospho_Antibody_022522](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A17020B_DB-HRP_RPS6-Phospho_Antibody_022522.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg protein) from serum-starved NIH/3T3 cells treated without (-) or with (+) 100 ng/mL Recombinant Mouse PDGF-BB (Cat. No. 558804) for 20 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with a 1:2000 dilution of Direct-Blot™ HRP anti-RPS6 Phospho (Ser235/Ser236) (clone A17020B). Proteins were visualized by chemiluminescence detection. Equal protein loading was confirmed using purified anti-RPS6 (Cat. No. 643807) and Direct-Blot™ HRP anti-β-actin (Cat. No. 643807). Lane M: molecular weight ladder
Cat # | Size | Price | Quantity Check Availability | Save | ||
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608611 | 25 µL | £73 | ||||
608612 | 100 µL | £233 |
Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the 7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabeteic mouse models.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:500 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Due to complete conservation of the immunizing sequence between humans, mouse and rat, this clone is is predicted to react with rat RPS6 phosphorylated at serines 235 and 236.
- RRID
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AB_2910485 (BioLegend Cat. No. 608611)
AB_2910485 (BioLegend Cat. No. 608612)
Antigen Details
- Structure
- RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
- Distribution
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Cytosol, nucleus, ER, ubiquitous expression.
- Function
- Phosphorylation events at a cluster of serine residues (Ser 235, 236, 240, and 244) at the carboxy-terminus of RPS6 results in increased global translational efficiency. These sites are dephosphorylated during growth arrest.
- Interaction
- RPS6AK1 and RPS6AK3; other subunits of 40S ribosomal complex.
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
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- Jefferies HB, et al. 1997. EMBO J. 16:3693.
- Ruvinsky I, et al. 2005. Genes. Dev.19:2199.
- Chumacher AM, et al. 2006. Biochemistry. 45:13614
- Roux PP, et al. 2007. J. Biol. Chem. 282:14056.
- Stevens C, et al. 2009. J. Biol. Chem. 284:334.
- Schlafli P, et al. 2011. FEBS J. 278:1757.
- Gene ID
- 6194 View all products for this Gene ID
- UniProt
- View information about RPS6 Phospho Ser235/Ser236 on UniProt.org
Related Pages & Pathways
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