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FITC anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_FITC_061407
Human peripheral blood monocytes stained with HCD14 FITC
  • HCD14_FITC_061407
    Human peripheral blood monocytes stained with HCD14 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
325603 25 tests £65
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325604 100 tests £145
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
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  23. Zhu H, et al. 2014. Lupus. 23:624. PubMed
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  28. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  29. An L, et al. 2016. Sci Rep. 6: 33346. PubMed
  30. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  31. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
  32. Ramaseri Sunder S, et al. 2020. J Interferon Cytokine Res. 40:310. PubMed
  33. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
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  35. Shen X, et al. 2020. Molecular Cell. 80(6):1013-1024.e6. PubMed
  36. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  37. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  38. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  39. Kerr SC, et al. 2020. Clin Exp Allergy. 50:904. PubMed
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RRID
AB_830676 (BioLegend Cat. No. 325603)
AB_830677 (BioLegend Cat. No. 325604)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

There are no FAQs for this product.
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