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FITC anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_FITC_122107
C57BL/6 bone marrow cells stained with TER-119 FITC and anti-mouse CD45 APC
  • TER-119_FITC_122107
    C57BL/6 bone marrow cells stained with TER-119 FITC and anti-mouse CD45 APC
See FITC spectral data
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116205 50 µg £29
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116206 500 µg £91
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  2. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
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  4. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
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  6. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  7. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  8. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  9. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  10. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  11. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  12. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  13. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  14. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
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  20. Ju W, et al. 2020. J Biomed Sci. 27:91. PubMed
  21. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  22. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  23. Guarnerio J, et al. 2015. Cancer Discovery. 5:396. PubMed
  24. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  25. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  26. Agarwal S, et al. 2015. Cell Rep. 13: 2147-2158. PubMed
  27. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
  28. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  29. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  30. Grandl G, et al. 2016. Mol Metab. 5:937-947. PubMed
  31. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  32. Koh BI, et al. 2020. Nat Commun. 3.143055556. PubMed
  33. Crispin A, et al. 2020. J Clin Invest. 130:5245. PubMed
  34. Kim DJ, et al. 2020. Proc Natl Acad Sci U S A. 117:15837. PubMed
  35. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  36. Marjanovic ND, et al. 2020. Cancer Cell. 38(2):229-246.e13. PubMed
  37. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
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  39. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  40. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  41. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
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  43. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  44. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
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  49. Wang X, et al. 2022. Elife. 11:. PubMed
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  56. Krishack PA, et al. 2021. Am J Respir Cell Mol Biol. 64:569. PubMed
  57. Jiang X, et al. 2021. Sci Signal. 14:. PubMed
  58. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  59. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  60. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
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  62. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  63. Liang Z, et al. 2022. iScience. 25:105233. PubMed
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  65. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  66. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  67. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
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RRID
AB_313706 (BioLegend Cat. No. 116205)
AB_313707 (BioLegend Cat. No. 116206)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org
Go To Top Version: 2    Revision Date: 12/04/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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