ELISA MAX™ Standard Set Human IL-6

Kit Contents

Kit Contents

• Human IL-6 ELISA MAX™ Capture Antibody (200X)
• Human IL-6 ELISA MAX™ Detection Antibody (200X)
• Human IL-6 Standard
• Avidin-HRP (1000X)

Product Details

Reactivity

Human

Application

ELISA

 

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Application References

(PubMed link indicates BioLegend citation)

1. Ishola Jr DA, et al. 2006. Kidney International 2006; doi:10.1038/sj.ki.5001629PubMed
2. Kim I, et al. 2006. Blood doi:10.1182/blood-2006-04-014399
3. Wimmer A, et al. 2006. Blood 108:3722.
4. Inokuchi J, et al. 2009. Carcinogenesis. 30:1028. PubMed
5. Hirari M, et al. 2011. Blood 117:500. PubMed
6. Zhou J, et al. 2015. Cytokine. 71:89. PubMed

Product Citations

1. Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed
2. Nieto C, et al. 2018. Front Immunol. 9:31. PubMed
3. Zhao M, et al. 2018. Cell Death Dis. 9:582. PubMed
4. Elizondo–Montemayor L, et al. 2019. Mediators Inflamm. 2019:6737318. PubMed
5. Bougarne N, et al. 2019. Front Immunol. 10:1769. PubMed
6. Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
7. Inokuchi J, et al. 2009. Carcinogenesis. 30:1082. PubMed
8. Hirai M, et al. 2011. Blood. 117:500. PubMed
9. Zhou J, et al. 2015. Cytokine. 71:89. PubMed
10. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
11. Gorby C, et al. 2020. Sci Signal. :13. PubMed
12. Seery V, et al. 2021. EBioMedicine. 67:103357. PubMed
13. Hoang QTM, et al. 2021. Front Med (Lausanne). 8:676058. PubMed
14. Ban T, et al. 2021. Nat Commun. 12:4379. PubMed
15. He X, et al. 2021. Cell Rep. 36:109392. PubMed
16. Stempin CC, et al. 2021. Cancers (Basel). 13:. PubMed
17. Karhadkar TR, et al. 2021. J Pharmacol Exp Ther. 376:106. PubMed
18. Yang JH, et al. 2021. Molecules. 26:. PubMed
19. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
20. Sohail A, et al. 2022. PLoS Pathog. 18:e1010219. PubMed
21. Zhang L, et al. 2022. Nat Commun. 13:360. PubMed
22. Chen S, et al. 2021. Exp Ther Med. 22:1049. PubMed
23. Gonzalez-Torres S, et al. 2021. Int J Environ Res Public Health. 19:. PubMed
24. Kirolos SA, et al. 2022. J Leukoc Biol. 112:1399. PubMed
25. Asano T, et al. 2022. J Clin Lipidol. 17:78. PubMed

Standard Range

7.8-500 pg/mL

Materials Not Included

• Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
• A microplate reader capable of measuring absorbance at 450 nm
• Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
• Deionized (DI) water
• Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
• Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
• PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
• Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
• Wash bottle or automated microplate washer
• TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
• Stop Solution (2 N H₂SO₄)
• Log-Log graph paper or software for data analysis
• Tubes to prepare standard dilutions
• Timer
• Absorbent paper

Antigen Details

Cell Sources

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Gene ID

3569 View all products for this Gene ID

UniProt

View information about IL-6 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis