Mouse MCP-1 ELISA MAX Standard

Pricing & Availability

Regulatory Status: RUO
Other Names: Mouse MCP-1 ELISA Kit
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Product Citations: publications

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432701

5 plates

£182

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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details

Technical Data Sheet (pdf)

Kit Contents

Kit Contents

Mouse MCP-1 ELISA MAX™ Capture Antibody (200X)
Mouse MCP-1 ELISA MAX™ Detection Antibody (200X)
Mouse MCP-1 Standard
Avidin-HRP (1000X)

Product Details

Reactivity

Mouse

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Product Citations

Sun H, et al. 2018. J Lipid Res. 59:207. PubMed
Miranda K, et al. 2018. Int J Obes (Lond). 42:1140. PubMed
Cárdenas–Torres FI, et al. 2019. Medicina (Kaunas). 55:3. PubMed
Bougarne N, et al. 2019. Front Immunol. 10:1769. PubMed
Zhou T, et al. 2019. Nat Neurosci. 22:421. PubMed
Santos I, et al. 2020. Proc Natl Acad Sci U S A. 117:12281. PubMed
He X, et al. 2021. Cell Rep. 36:109392. PubMed
Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
Lajqi T, et al. 2021. Int J Mol Sci. 22:. PubMed
Nagree MS, et al. 2023. Commun Biol. 6:560. PubMed

Standard Range

62.5-4,000 pg/mL

Materials Not Included

Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
Wash bottle or automated microplate washer
TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
Stop Solution (2 N H₂SO₄)
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Absorbent paper

Antigen Details

Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Biology Area: Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 20296 View all products for this Gene ID
UniProt: View information about CCL2 on UniProt.org