- Clone
- 6D5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B4
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6D5_Blue_091207 6D5_Blue_091207](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6D5_Blue_091207.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
115526 | 25 µg | £65 | ||||
115523 | 100 µg | £148 |
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD19-expressing K562 human erythroleukemia cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence7.
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Application References
(PubMed link indicates BioLegend citation) -
- Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
- Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
- Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
- Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
- Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
- Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
- Olive V, et al. 2013. Elife. 2:822. PubMed
- Miyai T, et al. 2014. PNAS. 111:11780. PubMed
- Product Citations
- RRID
-
AB_493341 (BioLegend Cat. No. 115526)
AB_439718 (BioLegend Cat. No. 115523)
Antigen Details
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Function
- Modulates B cell activation and differentiation
- Ligand/Receptor
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437. - Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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