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PE anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_PE_DYKDDDDK_Antibody_FC_022814.jpg
F-SIK2-FLAG transfected HEK293 cells were fixed and permeabilized with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), then stained with anti-DYKDDDDK (clone L5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • L5_PE_DYKDDDDK_Antibody_FC_022814.jpg
    F-SIK2-FLAG transfected HEK293 cells were fixed and permeabilized with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), then stained with anti-DYKDDDDK (clone L5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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637309 25 µg £81
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637310 100 µg £247
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Epitope tag
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Gao X, et al. 2018. Sci Rep. 8:2730. PubMed
  2. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  3. Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed
  4. Csizmar CM, et al. 2018. Bioconjug Chem. 29:1291. PubMed
  5. Lotta LA et al. 2019. Cell. 177(3):597-607 . PubMed
  6. Golec E, et al. 2019. FASEB J. 33:12425. PubMed
  7. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  8. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  9. Nagareddy B, et al. 2020. J Biol Chem. 295:13887. PubMed
  10. Liao Q, et al. 2020. Biomark Res. 8:57. PubMed
  11. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  12. Tong P, et al. 2021. Cell. :. PubMed
  13. Gao X, et al. 2020. J Biol Chem. 14893:295. PubMed
  14. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  15. Navare AT, et al. 2020. bioRxiv. . PubMed
  16. Li Q, et al. 2021. Cell. . PubMed
  17. Palma-Barqueros V, et al. 2020. Am J Hematol. . PubMed
  18. Okamoto Y, et al. 2021. J Cell Sci. 134:. PubMed
  19. He H, et al. 2021. J Immunother Cancer. 9:. PubMed
  20. Shao Z, et al. 2022. Nat Chem Biol. 18:264. PubMed
  21. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  22. Sabatino JJ, et al. 2022. JCI Insight. 7:. PubMed
  23. Galli A, et al. 2022. Virus Evol. 8:veab106. PubMed
  24. Bo Q, et al. 2022. Cell Discov. 8:47. PubMed
  25. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  26. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  27. Taft JM, et al. 2022. Cell. 185:4008. PubMed
  28. Navare AT, et al. 2022. J Cell Biol. 221: . PubMed
  29. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  30. Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed
  31. Zhu I, et al. 2022. Cell. 185:1431. PubMed
  32. Li S, et al. 2022. Elife. 11:. PubMed
  33. Chen C, et al. 2023. Cell Biochem Funct. 41:355. PubMed
  34. Silva RP, et al. 2023. Elife. 12:. PubMed
  35. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  36. Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed
RRID
AB_2563147 (BioLegend Cat. No. 637309)
AB_2563148 (BioLegend Cat. No. 637310)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 09/21/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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