- Clone
- 16E10A23 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![16E10A23_PE_GATA3_Antibody_FC_1_102813 16E10A23_PE_GATA3_Antibody_FC_1_102813](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16E10A23_PE_GATA3_Antibody_FC_1_102813.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human T leukemia cell line Jurkat (filled histogram) or Burkitt's lymphoma cell line Ramos (open histogram) was treated with FOXP3 Fix/Perm Buffer Set, and then stained with GATA3 (clone 16E10A23) PE. -
Murine Th2 cell line D10.G4.1 (filled histogram) and B lymphoma cell line A20 (open histogram) were treated with FOXP3 Fix/Perm Buffer Set, and then stained with GATA3 (clone 16E10A23) PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
653803 | 25 tests | £97 | ||||
653804 | 100 tests | £230 |
GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines, responsible for preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defect in GATA3 results in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human GATA3 recombinant protein (1-258 aa).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
- RRID
-
AB_2562722 (BioLegend Cat. No. 653803)
AB_2562723 (BioLegend Cat. No. 653804)
Antigen Details
- Structure
- 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.
- Distribution
-
Nucleus
- Function
- GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation.
- Interaction
- Interacts with Smad3, FOG1, FOG2, and PU.1.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Yagi R, et al. 2011. Int. Immunol. 23:415.
2. Chou J, et al. 2010. J. Cell Physiol. 222:42.
3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125.
4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236.
5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164.
6. Van Esch H, et al. 2000. Nature 406:419. - Gene ID
- 14462 View all products for this Gene ID 2625 View all products for this Gene ID
- UniProt
- View information about GATA3 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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